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WG 5 – Lateral and axial resolution

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WG 5 – Objectives

This WG focuses on the microscope lateral and axial resolution, which is essential for reporting size measurements of near- resolution limit objects or distances between them. Resolution is highly related to the objective quality but depends strongly upon other parameters ranging from the sample preparation to the signal detection.

The WG aims to define sample preparation, image acquisition, and data analysis protocols for testing resolution, first using sub-resolution fluorescent bead preparations and second employing alternative pattern-based methods. Monitoring the resolution (Point Spread Function in the case of beads) over time will identify possible aberrations in the system. Pooling the data from multiple laboratories within the WG will allow them to compare reproducibility for sample preparation, data acquisition, and data analysis tools, thereby determining a robust, easy-to-use protocol to propose to the community.

WG 5 – Co-Chairs

WG 5 - Latest News

QUAREP-LiMi WG5 Member Hosts Microscopy Workshop at Stellenbosch University

In April 2025, Yury Belyaev from the Microscopy Imaging Center (MIC) at the University of Bern, and a member of QUAREP-LiMi Working Group 5 (WG5), visited the Central Analytical Facilities (CAF) Microscopy Unit at Stellenbosch University, South Africa, as part of the Global BioImaging (GBI) International Job Shadowing Program

During his visit, Yury Belyaev collaborated with Lize Engelbrecht, manager of the CAF Microscopy Unit, to organize a hands-on microscopy workshop. The training covered wide-field and confocal microscopy, image analysis, and microscope maintenance. The event was generously supported by the CoRE Genomics for Health in Africa Scientific Exchange Program and Chroma

The workshop brought together participants from across South Africa and provided an excellent platform for Yury to showcase QUAREP-LiMi's activities, along with other international microscopy and image processing initiatives. A dedicated maintenance day also allowed for the presentation of protocols developed by QUAREP-LiMi, particularly those from WG5 in which Yury is actively involved.

QUAREP-LiMi welcomes our first members from Israel and Taiwan

The Working Group 5 - Lateral and Axial Resolution has recently welcomed a new member from Israel, Mr. Joseph Steinberger from the distinguished Weizmann Institute of Science. On behalf of the team, we extend a cordial welcome to him and we look forward to his active participation in our future endeavors.

We are pleased to announce a new addition to our QUAREP-LiMi community, Mr. Wei-Chen Chu from Academia Sinica in Taiwan.
We welcome him as we strive towards advancing the quality of light microscopy imaging on a global scale. We encourage like-minded professionals from all corners of the world to join us. We invite you to peruse our website for insights on how you can actively contribute and make a difference. Together, we can achieve remarkable outcomes, and contribute to the evolution of light microscopy imaging in the future.

First protocol for Monitoring the point spread function

The WG 5 released its first protocol “Monitoring the point spread function for quality control of confocal microscopes“. The protocol is featured on

 

Last meeting: 15th May 2025, 3:30 pm

Release of protocol “Monitoring the point spread function for quality control of confocal microscopes“.

49th meeting, 15th of May 2025

Meeting date

15/05/2025 @ 3:30 PM (CET/CEST)

Meeting documentation

48th meeting, 14th of April 2025

Meeting date

14/04/2025 @ 3:30 PM (CET/CEST)

Meeting summary

The protocol V2 was discussed and it was voted to pass the next version of the protocol to the SC. For the upcoming ELMI workshop, plans were initiated to host a one-hour session, considering point source and line patterns, and coordinating tasks among participants. An update on the paper shared insights on confocal SBR and r² values, noting differences due to noise factors. Discussions included data analysis techniques and the challenges of data variance over time. The meeting concluded with a call for organizing data in OMERO and an update on a new ISO norm for metadata, inviting community participation. The next meeting is postponed by a week to accommodate a French national holiday.

Meeting documentation

47th meeting, 13th of March 2025

Meeting date

13/03/2025 @ 3:30 PM (CET/CEST)

Meeting documentation

46th meeting, 13th of February 2025

Meeting date

13/02/2025 @ 3:30 PM (CET/CEST)

Meeting documentation

45th meeting, 9th of January 2025

Meeting date

09/01/2025 @ 3:30 PM (CET/CEST)

Meeting documentation

44th meeting, 14th of November 2024

Meeting date

14/11/2024 @ 3:30 PM (CET/CEST)

Meeting documentation

43rd meeting, 10th of October 2024

Meeting date

10/10/2024 @ 3:30 PM (CET/CEST)

Meeting documentation

42nd meeting, 19th of September 2024

Meeting date

14/09/2024 @ 3:30 PM (CET/CEST)

Meeting documentation

41st meeting, 11th of July 2024

Meeting date

11/07/2024 @ 3:30 PM (CET/CEST)

Meeting documentation

40th meeting, 13th of June 2024

Meeting date

13/06/2024 @ 3:30 PM (CET/CEST)

Meeting summary

We discussed the ELMI workshop, including how it went and what we could improve. We also covered the process of OMERO uploads and linking them to Airtable for tracking results, as well as the filenaming nomenclature.

Meeting documentation

Memberlist WG 5

Quality Assessment and Reproducibility for Instruments & Images in Light Microscopy

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