WG 13 - Objectives
In live fluorescence microscopy, phototoxicity describes the phenomenon by which the light used for fluorescence excitation leads to physiological changes in the observed living sample. Fluorescence imaging itself can change the physiology, and the conclusions drawn from such observations can be erroneous and misleading: Phototoxicity may cause subtle changes within the sample that are not easy to notice visually, and researchers may observe a physiological process for the first time, for which “normal” behaviour is not known. The goal of the phototoxicity working group (WG13) is to raise awareness of phototoxicity in the wider imaging community and share the best ways to assess, minimise and report it. Key aims of WG13 are:
- To bring together the international microscopy community to raise awareness about the issue of phototoxicity in live fluorescence microscopy. This includes imaging scientists working in core facilities, researchers and trainees (graduate students, postdoctoral fellows), scientific publishers, manuscript/grant reviewers, and manufacturers of optical instruments and software.
- The WG members will generate content and give phototoxicity workshops and short talks at different scientific meetings in their regional area and in their scientific field (e.g. biophysics, neuroscience…). WG13 will also periodically produce educational material (blog articles, videos, reviews) with up-to-date references (peer-reviewed publications, white papers and industry notes) regarding phototoxicity.
- To develop and disseminate general recommendations and best practices on how to measure, mitigate and report phototoxicity. For example, how to produce dose–response curves, how to avoid illumination overhead, and what biological readouts to use.
- To establish a consensus on phototoxicity-related terminology. For these points, we intend to work with the Microscopy Publication Standards group (WG11).
- To work with the Illumination Power group (WG1) to encourage researchers to measure incident light power for all live cell experiments.
- To collaborate with industry partners for the promotion of hardware settings and techniques that verifiably enable live imaging with low phototoxicity.
- To obtain funding that enables quantitative studies, educational activities and community building centered on phototoxicity.
WG 13 - Co-Chairs
Call:
+1 514-398-4400
Email:
claire.brown@mcgill.ca
Dr. Claire Brown
Co-Chair of BioImaging North America (BINA)
President of Canadian Network of Scientific Platforms (CNSP)
Chair of Canada BioImaging (CBI)
Co-Chair of WG 13
WG 13 - Latest News
Last meeting: 25th May 2022, 4:00 pm
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Memberlist WG 13
Last name First Name Town Organisation Affiliation Lab Affiliation Country Mail address Time Zone
Ali
Rizwan
Riyadh
KAIMRC
Univ.
SA
rizwanali79@gmail.com
UTC+3
Avilov
Sergiy
Freiburg
MPI Freiburg
MPG
DE
avilov@ie-freiburg.mpg.de
UTC+2
Brown
Claire
Montreal
BINA
Univ.
CA
claire.brown@mcgill.ca
UTC-4
Bruckbauer
Andreas
London
Imperial College London
Univ.
GB
a.bruckbauer@imperial.ac.uk
UTC+-0
Campbell
George
Memphis
St. Jude Children’s Research Hospital
Private lab
US
g.campbell.molecular@gmail.com
UTC-6
Carvalho
Mariana
Braga
INL – International Iberian Nanotechnology Laboratory
EU-Org
PT
mariana.carvalho@inl.int
UTC+-0
Chacko
Jenu
Madison
University of Wisconsin Madison
Univ.
US
jenu.chacko@wisc.edu
UTC-6
Drent
Peter
Amsterdam
ONI
Industry
NL
pdrent@oni.bio
UTC+1
Gaudreault
Nathalie
Seattle
BINA
Private lab
US
nathalieg@alleninstitute.org
UTC-7
Gibbs
Holly
College Station
Texas A&M University
Univ.
US
hgibbs@tamu.edu
UTC-6
Grunwald
David
Worcester
Univ. of Massachusetts
Univ.
US
david.grunwald@umassmed.edu
UTC-4
Hammer
Mathias
Worcester
University of Massachusetts Medical School
Univ.
US
mathias.hammer@umassmed.edu
UTC+5
Hatto
Mao
Praha 5
Nikon
Industry
CZ
mao.hatto@nikon.com
UTC+2
Hibbs
Ben
Silvan
Klein Australia Pty Ltd
Industry
AU
ben@kleinaustralia.com.au
UTC+10
Laissue
Philippe
Colchester
University of Essex
Univ.
GB
plaissue@essex.ac.uk
UTC+-0
Nitschke
Roland
Freiburg
GerBi-GMB WG 1
Univ.
DE
Roland.Nitschke@uni-freiburg.de
UTC+2
Porter
Iain
Dundee
University of Dundee
Univ.
GB
i.m.porter@dundee.ac.uk
UTC+-0
Rezgui
Rachid
Abu Dhabi
NYU Abu Dhabi
Univ.
AE
rachid.rezgui@nyu.edu
UTC+4
SALLES
Audrey
Paris
Institut Pasteur
Private lab
FR
audrey.salles@pasteur.fr
UTC+1
Srinivasan
Sathya
Beaverton
ONPRC
Univ.
US
scitecheditor@gmail.com
UTC-8
Tassis
Kostas
Leiden
Leiden University
Univ.
NL
k.tassis@lacdr.leidenuniv.nl
UTC+1
Tsuchiya
Takanori
Fujinomiya
TOKAI HIT
Industry
JP
t-tsuchiya@tokaihit.com
UTC+9
Vogl
Ulrich
Oberkochen
Carl Zeiss AG
Industry
DE
ulrich.vogl@zeiss.com
UTC+1
Last name First Name Town Organisation Affiliation Lab Affiliation Country Mail address Time Zone