Public Protocols
All protocols are available on protocols.io
2023
2pth March
Illumination Power, Stability and Linearity Measurements for Confocal and Widefield Microscopes V.2
Authors:
Nathalie Gaudreault1, Rizwan Ali2, Sergiy V Avilov3, Steve Bagley4, Rodrigo R Bammann5, Fabio Barachati6, Laszlo Barna7, Valeria Berno8, Cristina Bertocchi9, Veronika Boczonadi10, Ulrike Boehm11, Manel Bosch12, Craig Brideau13, Mariana T Carvalho14, Nabanita Chatterjee15, Pina Colarusso16, Richard Cole17, Nasser Darwish-Miranda18, Suat Dervish19, Ralf Dietzel20, Sam Duwé21, Frank Eismann22, Orestis Faklaris23, Andreas Felscher24, Zannatul Ferdous25, Thomas Guilbert26, Manfred Gonnert27, David Grunwald28, Birgit Hoffmann29, Marcel Kirchner30, Ayse Aslihan Koksoy31, Gabriel Krens18, Alex J Laude10, Jeffrey M LeDue32, Penghuan Liu33, Pascal Lorentz34, Gabriel G Martins35, Valeria.MezzanoRobinson36, Miso Mitkovski37, Glyn Nelson38, Michael S Nelson39, Roland Nitschke40, Alex L Payne-Dwyer41, Jaime Arturo Pimentel Cabrera42, Santosh Podder43, Britta Schroth-Diez44, Stanley Schwartz45, Sathya Srinivasan46, Roland Thuenauer47, Kees van der Oord48, Douglas J Taatjes49, Chloë van Oostende-Triplet50, Tse-Luen Wee51, Kurt R Weiss52, Laurent Gelman53
1Allen Institute for Cell Science, Seattle, WA, USA; 2King Abdullah International Medical Research Center/ King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), Saudi Arabia; 3Max Planck Institute of Immunobiology and Epigenetics, Germany; 4Cancer Research UK Manchester Institute, UK; 5Scientifica; 6PicoQuant GmbH; 7Institute of Experimental Medicine, Budapest, Hungary; 8Milano, Italy; 9Pontificia Universidad Católica de Chile, Chile; 10Newcastle University, BioImaging Unit, UK; 11Carl Zeiss AG, Oberkochen, Germany; 12Scientific and Technological Centers, Universitat de Barcelona (CCiTUB), Spain; 13University of Calgary, Canada; 14INL – International Iberian Nanotechnology Laboratory; 15UNIVERSITY OF GOTHENBURG Centre for Cellular Imaging – Core Facilities Sahlgrenska Academy, Sweden; 16Live Cell Imaging Laboratory, Snyder Institute of Chronic Diseases, University of Calgary, Canada; 17New York State Dept of Health, Wadsworth Center, USA; 18Institute of Science and Technology Austria; 19Westmead Cytometry, Imaging, & Electron Microscopy, Sydney, Australia; 20Omicron Laserage Laserprodukte GmbH, Germany; 21Advanced Optical Microscopy Centre; 22Carl Zeiss Microscopy GmbH, Germany; 23MRI, Biocampus, University of Montpellier, CNRS, INSERM, Montpellier, France; 24Coherent GmbH & Co.KG; 25Hokkaido University; 26Institut Cochin, Inserm, France; 27Thorlabs GmbH; 28UMass Chan Medical School, USA; 29University Hospital Jena, Biomolecular Photonics Group; 30Max Planck Institute for Biology of Ageing, Germany; 31MD Anderson Cancer Center; 32University of British Columbia, Canada; 33Key Laboratory for Modern Measurement Technology and Instruments of Zhejiang Province, China Jiliang University, Hangzhou, China; 34Department of Biomedicine, University of Basel, Zwitzerland; 35Advanced Imaging – Inst. Gulbenkian de Ciencia, Portugal; 36Experimental Pathology, NYU Sch. Of Medicine, USA; 37Max Planck Institute for Multidisciplinary Sciences; 38Bioimaging Unit, Newcastle University, UK; 39Department of Biomedical Engineering, University of Wisconsin – Madison, USA; 40Life Imaging Center, University of Freiburg, Germany; 41School of Physics, University of York,; 42Instituto de Biotecnología. Universidad Nacional Autónoma de México; 43Indian Institute of Science Education and Research (IISER) Pune; 44Max Planck Insitute of Molecular Cell Biology and Genetics, Dresden, Germany; 45ISO International Standards Organization TC172 SC5; 46Oregon National Primate Research Center (ONPRC, OHSU West Campus), USA; 47Leibniz Institute for Experimental Virology (HPI), Germany; 48Nikon Europe BV; 49Microscopy Imaging Center, University of Vermont, Burlington, VT USA; 50Cell Biology and Image Acquisition Core Facility, University of Ottawa, Canada; 51Cold Spring Harbor Laboratory, USA; 52UW Madison Biochemistry Dept.; 53Friedrich Miescher Institute for Biomedical Research, Switzerland
DOI: dx.doi.org/10.17504/protocols.io.5jyl853ndl2w/v2
2022
19th October
Monitoring the point spread function for quality control of confocal microscopes
Authors:
Glyn Nelson1, Ioannis Alexopoulos2, Maria Azevedo3, Fabio Barachati4, Yury Belyaev5, Mariana T Carvalho6, Yann Cesbron7, Aurelien Dauphin8, Alexander D Corbett9, Ian M Dobbie10, Laurent Gelman11, Nadia Halidi12, Xiang Hao13, Hella Hartmann14, Rainer Heintzmann15, Peter Hemmerich16, Marcel Kirchner17, Judith Lacoste18, Penghuan Liu19, Laure lantard20, Claire A Mitchell21, Tobias Müller22, Kees van der Oord23, Alex L Payne-Dwyer24, Santosh Podder25, Rachid Rezgui26, Sandra Ritz27, Stanley Schwartz28, Michael J Shaw29, Tse-Luen Wee30, Roland Nitschke31, Orestis Faklaris32
1Bioimaging Unit, Newcastle University, UK; 2Multiscale Imaging Platform, Institute for Lung Health, Justus Liebig Universität Gießen; 3i3S – Instituto de Investigação e Inovação em Saúde, University of Porto, Portugal; 4PicoQuant GmbH; 5Microscopy Imaging Center (MIC), University of Bern, Bern, Switzerland; 6INL – International Iberian anotechnology Laboratory; 7Institute of Science and Technology Austria, Klosterneuburg, Austria; 8Unite Genetique et Biologie du Développement U934, PICT-IBiSA, Institut Curie, INSERM, CNRS, PSL Research University, Paris, 75005, France; 9University of Exeter; 10Johns Hopkins University; 11Friedrich Miescher Institute for Biomedical Research; 12Centre for Genomic Regulation; 13Zhejiang University, Hangzhou 310027, China; 14TU Dresden, CMCB; 15Leibniz Institute of Photonic Technology; 16Leibniz Institute on Aging, Core Facility Imaging, Jena, Germany; 17Max Planck Institute , Koln; 18MIA Cellavie Inc.; 19College of Optical and Electronic Technology, China Jiliang Univeristy, Hangzhou, hina; 20Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland; 21Beatson Advanced Imaging Resource, The Beatson Institute for Cancer Research, Glasgow, UK; 22IMP, Vienna; 23Nikon Europe BV; 24School of Physics, Engineering and Technology, University of York, UK; 25Indian Institute of Science Education and Research (IISER) Pune; 26New York University, Abu Dhabi; 27Institute of Molecular Biology gGmbH (IMB); 28ISO International Standards Organization TC172 SC5; 29National Physical Laboratory; 30Cold Spring Harbor Laboratory; 31Life Imaging Center, University of Freiburg, Germany; 32MRI, Biocampus, University of Montpellier, CNRS, INSERM, Montpellier, France
DOI: dx.doi.org/10.17504/protocols.io.bp2l61ww1vqe/v1
2022
19th October
Repeatability of the XY positioning of motorized xy-tables of light microscopes
Authors:
Mariana T Carvalho1, Hans Fried2, Stuart Jarvis3, Claire A Mitchell4, Roland Nitschke5, Kees van der Oord6, Arnd Rühl7, Stanley Schwartz8, Martin Spitaler9, Roman Zantl10, Arne Seitz
1INL – International Iberian Nanotechnology Laboratory; 2CRFS – Light Microscope Facility, German Center for Neurodegenerative Diseases (DZNE); 3Prior Scientific Instruments Limited; Beatson Advanced Imaging Resource, The Beatson Institute for Cancer Research, Glasgow, UK; 5Life Imaging Center, University of Freiburg, Germany; 6Nikon Europe BV; 7Märzhäuser Wetzlar; 8ISO International Standards Organization TC172 SC5; 9Max Planck Institute of Biochemistry; 10ibidi GmbH; 11Ecole Polytechnique Fédérale de Lausanne (EPFL)
DOI: dx.doi.org/10.17504/protocols.io.kxygxz2rdv8j/v1
2022
25th August
Getting started with Micro-Meta App Tutorial V.6
Authors:
Alessandro Rigano1, Mathias Hammer2, Joel Ryan3, Claire. Brown3, David Grunwald2, Caterina Strambio De Castillia1
1Program in Molecular Medicine, UMass Chan Medical School, Worcester, MA, USA; 2RNA Therapeutics Institute, UMass Chan Medical School, Worcester, MA, USA; 3Advanced BioImaging Facility (ABIF), McGill University, Montreal, Quebec, Canada
DOI: dx.doi.org/10.17504/protocols.io.36wgq7ddxvk5/v6
