Public Protocols
All protocols are available on protocols.io
2022
4th Feburary
Illumination Power and Illumination Stability
Authors:
Nathalie Gaudreault1, Steve Bagley2, Rodrigo R Bammann3, Fabio Barachati4, Laszlo Barna5, Veronika Boczonadi6, Ulrike Boehm7, Manel Bosch8, Craig Brideau9, Mariana T Carvalho10, Pina Colarusso11, Richard Cole12, Nasser Darwish-Miranda13, Sam Duwé14, Frank Eismann15, Orestis Faklaris16, Andreas Felscher17, Manfred Gonnert18, David Grunwald19, Marcel Kirchner20, Birgit Hoffmann21, Gabriel Krens13, Alex J Laude6, Jeffrey M LeDue22, Pascal Lorentz23, Miso Mitkovski24, Michael S Nelson25, Britta Schroth-Diez26, Stanley Schwartz27, Sathya Srinivasan28, Roland Thuenauer29, Tse-Luen Wee30, Kees van der Oord 31, Chloë van Oostende-Triplet32, Roland Nitschke33, Laurent Gelman34
1Allen Institute for Cell Science, Seattle, WA, USA; 2Cancer Research UK Manchester Institute; 3Scientifica; 4PicoQuant GmbH; 5Institute of Experimental Medicine, Budapest; 6Newcastle University, BioImaging Unit, UK; 7Janelia Research Campus, Howard Hughes Medical Institute; 8Scientific and Technological Centers, Universitat de Barcelona (CCiTUB); 9University of Calgary; 10INL – International Iberian Nanotechnology Laboratory; 11Live Cell Imaging Laboratory, Snyder Institute of Chronic Diseases, University of Calgary; 12New York State Dept of Health; 13Institute of Science and Technology Austria; 14Advanced Optical Microscopy Centre; 15Carl Zeiss Microscopy GmbH; 16MRI, Biocampus, University of Montpellier, CNRS, INSERM, Montpellier, France; 17Coherent GmbH & Co.KG; 18Thorlabs GmbH; 19UMass Chan Medical School; 20Max Planck Institute for Biology of Ageing; 21University Hospital Jena, Biomolecular Photonics Group; 22University of British Columbia; 23Department of Biomedicine, University of Basel; 24Max Planck Institute for Multidisciplinary Sciences; 25Department of Biomedical Engineering, University of Wisconsin – Madison; 26Max Planck Insitute of Molecular Cell Biology and Genetics; 27ISO International Standards Organization TC172 SC5; 28Oregon National Primate Research Center (ONPRC, OHSU West Campus); 29Leibniz Institute for Experimental Virology (HPI); 30Cold Spring Harbor Laboratory; 31Nikon; 32Cell Biology and Image Acquisition Core Facility, University of Ottawa; 33Life Imaging Center, University of Freiburg, Germany; 34Friedrich Miescher Institute for Biomedical Research
DOI: 10.17504/protocols.io.bzp8p5rw
2022
19th October
Monitoring the point spread function for quality control of confocal microscopes
Authors:
Glyn Nelson1, Ioannis Alexopoulos2, Maria Azevedo3, Fabio Barachati4, Yury Belyaev5, Mariana T Carvalho6, Yann Cesbron7, Aurelien Dauphin8, Alexander D Corbett9, Ian M Dobbie10, Laurent Gelman11, Nadia Halidi12, Xiang Hao13, Hella Hartmann14, Rainer Heintzmann15, Peter Hemmerich16, Marcel Kirchner17, Judith Lacoste18, Penghuan Liu19, Laure lantard20, Claire A Mitchell21, Tobias Müller22, Kees van der Oord23, Alex L Payne-Dwyer24, Santosh Podder25, Rachid Rezgui26, Sandra Ritz27, Stanley Schwartz28, Michael J Shaw29, Tse-Luen Wee30, Roland Nitschke31, Orestis Faklaris32
1Bioimaging Unit, Newcastle University, UK; 2Multiscale Imaging Platform, Institute for Lung Health, Justus Liebig Universität Gießen; 3i3S – Instituto de Investigação e Inovação em Saúde, University of Porto, Portugal; 4PicoQuant GmbH; 5Microscopy Imaging Center (MIC), University of Bern, Bern, Switzerland; 6INL – International Iberian anotechnology Laboratory; 7Institute of Science and Technology Austria, Klosterneuburg, Austria; 8Unite Genetique et Biologie du Développement U934, PICT-IBiSA, Institut Curie, INSERM, CNRS, PSL Research University, Paris, 75005, France; 9University of Exeter; 10Johns Hopkins University; 11Friedrich Miescher Institute for Biomedical Research; 12Centre for Genomic Regulation; 13Zhejiang University, Hangzhou 310027, China; 14TU Dresden, CMCB; 15Leibniz Institute of Photonic Technology; 16Leibniz Institute on Aging, Core Facility Imaging, Jena, Germany; 17Max Planck Institute , Koln; 18MIA Cellavie Inc.; 19College of Optical and Electronic Technology, China Jiliang Univeristy, Hangzhou, hina; 20Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland; 21Beatson Advanced Imaging Resource, The Beatson Institute for Cancer Research, Glasgow, UK; 22IMP, Vienna; 23Nikon Europe BV; 24School of Physics, Engineering and Technology, University of York, UK; 25Indian Institute of Science Education and Research (IISER) Pune; 26New York University, Abu Dhabi; 27Institute of Molecular Biology gGmbH (IMB); 28ISO International Standards Organization TC172 SC5; 29National Physical Laboratory; 30Cold Spring Harbor Laboratory; 31Life Imaging Center, University of Freiburg, Germany; 32MRI, Biocampus, University of Montpellier, CNRS, INSERM, Montpellier, France
DOI: dx.doi.org/10.17504/protocols.io.bp2l61ww1vqe/v1
2022
19th October
Repeatability of the XY positioning of motorized xy-tables of light microscopes
Authors:
Mariana T Carvalho1, Hans Fried2, Stuart Jarvis3, Claire A Mitchell4, Roland Nitschke5, Kees van der Oord6, Arnd Rühl7, Stanley Schwartz8, Martin Spitaler9, Roman Zantl10, Arne Seitz
1INL – International Iberian Nanotechnology Laboratory; 2CRFS – Light Microscope Facility, German Center for Neurodegenerative Diseases (DZNE); 3Prior Scientific Instruments Limited; Beatson Advanced Imaging Resource, The Beatson Institute for Cancer Research, Glasgow, UK; 5Life Imaging Center, University of Freiburg, Germany; 6Nikon Europe BV; 7Märzhäuser Wetzlar; 8ISO International Standards Organization TC172 SC5; 9Max Planck Institute of Biochemistry; 10ibidi GmbH; 11Ecole Polytechnique Fédérale de Lausanne (EPFL)
DOI: dx.doi.org/10.17504/protocols.io.kxygxz2rdv8j/v1
2022
25th August
Getting started with Micro-Meta App Tutorial V.6
Authors:
Alessandro Rigano1, Mathias Hammer2, Joel Ryan3, Claire. Brown3, David Grunwald2, Caterina Strambio De Castillia1
1Program in Molecular Medicine, UMass Chan Medical School, Worcester, MA, USA; 2RNA Therapeutics Institute, UMass Chan Medical School, Worcester, MA, USA; 3Advanced BioImaging Facility (ABIF), McGill University, Montreal, Quebec, Canada
DOI: dx.doi.org/10.17504/protocols.io.36wgq7ddxvk5/v6
