WG 5 – Lateral and axial resolution

We are pleased to announce that the QUAREP-LiMi consortium will be presenting five specialized workshops at the upcoming European Light Microscopy Institute (ELMI) meeting. Our sessions are designed to provide researchers with frameworks for improving image quality, ensuring data reproducibility, and implementing rigorous quality control standards in microscopy. The workshops will cover five critical pillars of instrument characterization:

1. The QUAREP-LiMi Tool Kit (WGs 1 & 2):

   Discover how to streamline periodic quality assessments. We will present the latest developments in the Tool Kit, including user-friendly software tools for illumination power and stability (compatible with Nikon and Zeiss), as well as its data browser for long-term performance monitoring.

2. Detector Performance & Photon Characterization (WG2):

   Learn how to transition from "arbitrary digital units" (ADU) to actual photon counts using the Photon Transfer Curve (PTC) method. We will demonstrate protocols for characterizing photon conversion factors, read noise, and dynamic range for both point and area detectors.

3. Assessing the co-registration accuracy of a microscope (WG4):

   Chromatic aberration is a major source of spatial error in multi‑colour fluorescence microscopy. This workshop will show practical hints and tips to perform routine co‑registration measurement and analysis.

4. Point Spread Function (PSF) Analysis (WG5):

   This workshop provides a practical guide to PSF measurement—from beads slide preparation and image acquisition to analysis using open-source tools and integration with OMERO for longitudinal monitoring.

5. The Light-Microscopy Metadata Model (LiMi-Model, WG7):

   In collaboration with BioImaging North America, 4D Nucleome, and OME, we will introduce the LiMi-Model. This framework aims to harmonize the description of hardware and acquisition settings to ensure datasets are FAIR (Findable, Accessible, Interoperable, and Reusable) by design.