WG 5 – Objectives
This WG focuses on the microscope lateral and axial resolution, which is essential for reporting size measurements of near- resolution limit objects or distances between them. Resolution is highly related to the objective quality but depends strongly upon other parameters ranging from the sample preparation to the signal detection.
The WG aims to define sample preparation, image acquisition, and data analysis protocols for testing resolution, first using sub-resolution fluorescent bead preparations and second employing alternative pattern-based methods. Monitoring the resolution (Point Spread Function in the case of beads) over time will identify possible aberrations in the system. Pooling the data from multiple laboratories within the WG will allow them to compare reproducibility for sample preparation, data acquisition, and data analysis tools, thereby determining a robust, easy-to-use protocol to propose to the community.
WG 5 – Co-Chairs
Call:
+44 191 208 4860
Email:
glyn.nelson@newcastle.ac.uk
Glyn Nelson
Co-Chair of WG 5
WG 5 - Latest News
In April 2025, Yury Belyaev from the Microscopy Imaging Center (MIC) at the University of Bern, and a member of QUAREP-LiMi Working Group 5 (WG5), visited the Central Analytical Facilities (CAF) Microscopy Unit at Stellenbosch University, South Africa, as part of the Global BioImaging (GBI) International Job Shadowing Program
During his visit, Yury Belyaev collaborated with Lize Engelbrecht, manager of the CAF Microscopy Unit, to organize a hands-on microscopy workshop. The training covered wide-field and confocal microscopy, image analysis, and microscope maintenance. The event was generously supported by the CoRE Genomics for Health in Africa Scientific Exchange Program and Chroma
The workshop brought together participants from across South Africa and provided an excellent platform for Yury to showcase QUAREP-LiMi's activities, along with other international microscopy and image processing initiatives. A dedicated maintenance day also allowed for the presentation of protocols developed by QUAREP-LiMi, particularly those from WG5 in which Yury is actively involved.
The Working Group 5 - Lateral and Axial Resolution has recently welcomed a new member from Israel, Mr. Joseph Steinberger from the distinguished Weizmann Institute of Science. On behalf of the team, we extend a cordial welcome to him and we look forward to his active participation in our future endeavors.
We are pleased to announce a new addition to our QUAREP-LiMi community, Mr. Wei-Chen Chu from Academia Sinica in Taiwan.
We welcome him as we strive towards advancing the quality of light microscopy imaging on a global scale. We encourage like-minded professionals from all corners of the world to join us. We invite you to peruse our website for insights on how you can actively contribute and make a difference. Together, we can achieve remarkable outcomes, and contribute to the evolution of light microscopy imaging in the future.
Last meeting: 9th April 2026, 3:30 pm
Release of protocol “Monitoring the point spread function for quality control of confocal microscopes“.
Memberlist WG 5
Last name First Name Town Organisation Affiliation Lab Affiliation Country Time Zone
Acevedo
Luis
Overland Park
thya
Industry
US
UTC-6
Ahmadpour
Noushin
Winnipeg
University of Manitoba
Univ.
CA
UTC-5
Akçınar
Salim Can
Izmir
Ege University
Univ.
TR
UTC+3
Alexopoulos
Ioannis
Giessen
ILH/CIGL Multiscale Imaging Platform
Univ.
DE
UTC+2
Alshafie
Walaa
Ashburn
Janelia research campus
Univ.
US
UTC-4
Avilov
Sergiy
Freiburg
Max Planck Institute of Immunobiology and Epigenetics
MPG
DE
UTC+2
Azevedo
Maria
Porto
i3S/University of Porto
Univ.
PT
UTC+1
Badrodien
Imraan
West Haven
Yale University
Univ.
US
UTC-4
Bagley
Steve
Manchester
University of Manch.
Univ.
GB
UTC+1
Banerjee
Priyam
New York
Rockefeller University
Univ.
US
UTC-5
Barachati
Fábio
Berlin
PicoQuant GmbH
Industry
DE
UTC+1
Barbu-Stevanovic
Mihaela
New York
New York Blood Center
Industry
US
UTC-6
Bassi
Andrea
Milano
Politecnico di Milano
Univ.
IT
UTC+2
Batti
Laura
Geneva
Wyss Center
Private lab
CH
UTC+1
Belyaev
Yury
Bern
University of Bern
Univ.
CH
UTC+1
Bertocchi
Cristina
Santiago de Chile
Pontificia Universidad Católica de Chile
Univ.
CL
UTC-3
Bierwagen
Jakob
Tuebingen
AHF
Industry
DE
UTC+2
Bosch
Manel
Barcelona
University of Barcelona CCITUB
Univ.
ES
UTC+2
Bottomley
Amy
Ultimo
University of Technology Sydney
Univ.
AU
UTC+10
Bourg
Nicolas
Cachan
Abbelight
Industry
FR
UTC+1
Bowman
Richard
Glasgow
University of Glasgow
Univ.
GB
UTC+-0
Boyce
Aidan
Cours-de-Monségur
Oxford Inst. Andor
Industry
FR
UTC+1
Broeking
Kai
Goettingen
MPI
MPG
DE
UTC+2
Bronze de Firmino
Joao Pedro
Hamilton
Centre for Advanced Light Microscopy at McMaster University
Univ.
CA
UTC-5
Brunt
Peter
Fairport
AVR Optics
Industry
US
UTC-5
Carvalho
Mariana
Braga
INL – International Iberian Nanotechnology Laboratory
EU-Org
PT
UTC+-0
Casse
Alhassan
Vitry-sur-Seine
SANOFI
Industry
FR
UTC+2
Catrina
Irina
New York
Yeshiva University
Univ.
US
UTC-5
Ceccarelli
Raino
Rome
CrestOptics S.p.A.
Industry
IT
UTC+1
Cesbron
Yann
Klosterneuburg
Institute of Science and Technology Austria
Gov.
AT
UTC+1
Chacko
Jenu
Madison
University of Wisconsin-Madison
Univ.
US
UTC-6
Cialowicz
Katarzyna
New York
Icahn School of Medicine at Mount Sinai
Univ.
US
UTC-5
Cole
Louise
Sydney
University of Technology
Univ.
AU
UTC+11
Corbett
Alex
Exeter
UK BioImaging
Univ.
GB
UTC+1
Cortes
Luisa
Coimbra
Center for Neuroscience and Cell Biology
Univ.
PT
UTC+0
Cresens
Charlotte
Leuven
VIB
Univ.
BE
UTC+2
Dauphin
Aurelien
Paris
RTmfm
Univ.
FR
UTC+2
De Keersmaecker
Herlinde
Ghent
UGent
Univ.
BE
UTC+1
De Niz
Mariana
Chicago
Northwestern University
Univ.
US
UTC-5
Dencevski
Aleksa
Belgrade
Institute of physics Belgrade
Univ.
CS
UTC+1
Dervish
Suat
Westmead
Westmead Institute for Medical Research
Gov.
AU
UTC+10
D’Este
Elisa
Heidelberg
Max Planck Institute for Medical Research
MPG
DE
UTC-1
Diez-Guerra
F. Javier
Madrid
CBMSO (CSIC-UAM)
Univ.
ES
UTC+2
Dobbie
Ian
Baltimore
Johns Hopkins University
Univ.
US
UTC+5
Dostál
Zbyněk
Brno
Brno University of Technology
Univ.
CZ
UTC+1
Drent
Peter
Amsterdam
ONI
Industry
NL
UTC+1
Duong
Cao Nguyen
Saarbrücken
Leibniz Institute for New Materials
Leibniz
DE
UTC+1
Duwé
Sam
Diepenbeek
Universiteit Hasselt
Univ.
BE
UTC+1
Dzelzainis
Thomas
Didcot
Rutherford Appleton Laboratory
Gov.
GB
UTC+-0
Eseola
Alice
Norwich
The Sainsbury Laboratory
Univ.
GB
UTC+1
Esner
Milan
Brno
Ceitec Masaryk University
Univ.
CZ
UTC+1
Faklaris
Orestis
Montpellier
RTmfm
Univ.
FR
UTC+2
Fallet
Mathieu
Marseille
CNRS
Univ.
FR
UTC+1
Fallisch
Arne
Freiburg
University of Freiburg, Life Imaging Center
Univ.
DE
UTC+1
Franke
Christian
Jena
Institute of Applied Optics and Biophysics of the Friedrich-Schiller-Universität Jena
Univ.
DE
UTC+2
Geißler
Daniel
Berlin
PolyAn GmbH
Industry
DE
UTC+1
Gelman
Laurent
Basel
Friedrich Miescher Institute for Biomedical Research
Private lab
CH
UTC+2
Goda
Kazuhito
Hachioji
EVIDENT
Industry
JP
UTC+9
Grunwald
David
Worcester
University of Massachusetts
Univ.
US
UTC-4
Guerrero
Adan
Cuernavaca
National Autonomous University of Mexico
Univ.
MX
UTC+6
Halidi
Nadia
Barcelona
Centre for Genomic Regulation
Gov.
ES
UTC+2
Hao
Xiang
Hangzhou
Zhejiang University
Univ.
CN
UTC+8
Harizanova
Jana
Copenhagen
CFIM/University of Copenhagen
Univ.
DK
UTC+1
Hartmann
Hella
Dresden
GerBi-GMB WG 1
Univ.
DE
UTC+2
Hassan
Ahmed
Thuwal
King Abdullah University of Sccience & Technology
Univ.
SA
UTC+3
Hassani
Ibrahim
Amsterdam
Nikon BV
Industry
NL
UTC+2
Hatto
Mao
Prague
Nikon
Industry
CZ
UTC+2
Hawkins
Tim
Durham
Durham University
Univ.
GB
UTC+-0
Heintzmann
Rainer
Jena
Friedrich Schiller University Jena
Univ.
DE
UTC+1
Hemmerich
Peter
Jena
GerBi-GMB WG 1
Leibniz
DE
UTC+2
Herzog
Dylan
London
Microscopy Innovation Centre, King’s College London
Univ.
GB
UTC+-0
Hibbs
Ben
Silvan
Klein Australia Pty Ltd
Industry
AU
UTC+10
Hoischen
Christian
Jena
Leibniz Institute on Aging – Fritz Lipmann Institute
Leibniz
DE
UTC+1
Inavalli
V V G
Southampton
University of Southampton
Univ.
GB
UTC+-0
Jiang
Junqing
London
National Physical Laboratory
Gov.
GB
UTC+-0
Jost
Aurélie
Jena
Friedrich Schiller University Jena
Univ.
DE
UTC+1
Kaiping
Arnica
Freiburg
University of Freiburg
Univ.
DE
UTC+2
Kapsokalyvas
Dimitris
Aachen
University Hospital RWTH Aachen University
Univ.
DE
UTC+1
Kirchenbüchler
David
Chicago
Northwestern
Univ.
US
UTC-6
Kirchner
Marcel
Cologne
MPI
MPG
DE
UTC+2
Köning
Rainer
Braunschweig
PTB Braunschweig
Gov.
DE
UTC+1
Kremer
Manuel
Mannheim
Leica Microsystems CMS GmbH
Industry
DE
UTC+2
Krens
Gabriel
Klosterneuburg
Institute of Science and Technology Austria
Gov.
AT
UTC+1
Krzic
Uros
Munich
Carl Zeiss Microscopy GmbH
Industry
DE
UTC+2
Kumar
Purnima
CAMBRIDGE
Gurdon Institute, University of Cambridge
Univ.
GB
UTC+-0
Kuo
Scot
Baltimore
Johns Hopkins University
Univ.
US
UTC-5
Lacoste
Judith
Montreal
MIA Cellavie Inc.
Industry
CA
UTC-4
Lagerholm
Christoffer
Oxford
University of Oxford
Univ.
GB
UTC+1
Lai
Pok Yui
Oxford
University of Oxford
Univ.
GB
UTC+1
Lai
Queenie
London
The Institute of Cancer Research
Univ.
GB
UTC+-0
Laktiushkin
Viktor
Sochi
Sirius
Univ.
RU
UTC+3
Landua
Kalika
Houston
Nikon Instruments
Industry
US
UTC-5
Lee
Kitty
Stanford
Stanford University
Univ.
US
UTC-8
Lees
Robert
Didcot
Science and Technology Facilities Council
Gov.
GB
UTC+-0
Levitt
James
London
Nikon Imaging Centre, King’s College London, UK
Univ.
GB
UTC+-0
Li
Xiaoming
Shanghaitech
Shanghaitech University
Univ.
CN
UTC+8
Liu
Penghuan
Hangzhou
China Jiliang University
Univ.
CN
UTC+8
Liu
Tristan
New York
Columbia University
Univ.
US
UTC-4
Louis
Boris
Leuven
KU Leuven
Univ.
BE
UTC+1
Madi
Mehdi
Cachan
Abbelight
Industry
FR
UTC+1
Manning
Lane
Fairport
AVR Optics
Industry
US
UTC-5
Marsal
Maria
Castelledefels
ICFO
Univ.
ES
UTC+1
Mast
Fred
Seattle
Seattle Children’s Hospital
Univ.
US
UTC+8
Metcalf
Daniel
Amsterdam
Nikon Europe
Industry
NL
UTC+1
Micu
Ileana
Belfast
Queen’s University Belfast
Univ.
GB
UTC+1
Mitchell
Claire
Glasgow
The Beatson Institute for Cancer Research
Univ.
GB
UTC+1
Monterroso
Baptiste
Nice
University of Nice
Univ.
FR
UTC+2
Montón Domingo
Helena
Bellaterra (Cerdanyola del Vallès)
Universitat Autònoma de Barcelona
Univ.
ES
UTC+1
Mulholland
Jonathan
Stanford
Stanford University
Univ.
US
UTC-7
Narvaez
William
Lastenia Toral
NGSI
Private lab
EC
UTC-5
Nechyporuk-Zloy
Volodymyr
London
Imperial College of Science, Technology and Medicine
Univ.
GB
UTC+-0
Nelson
Glyn
Newcastle
UK BioImaging
Univ.
GB
UTC+1
Nitschke
Roland
Freiburg
University of Freiburg, Life Imaging Center
Univ.
DE
UTC+2
Olifirov
Borys
Kyiv
Bogomoletz Institute of Physiology of NAS of Ukraine
Gov.
UA
UTC+2
Oord
Kees
Amsterdam
Nikon
Industry
NL
UTC+1
Oreopoulos
John
Toronto
Oxford Inst. Andor
Industry
CA
UTC-5
Orlova
Natalia
Seattle
Allen Institute
Private lab
US
UTC-7
Parel
Emma
Cape Town
University of Cape Town
Univ.
ZA
UTC+2
Payne-Dwyer
Alex
York
University of York
Univ.
GB
UTC+-0
Pereira
Telmo
Lisbon
CEDOC
Univ.
PT
UTC+-0
Perin
Paola
Pavia
University of Pavia
Univ.
IT
UTC+1
Peron-Cane
Caroline
PARIS
TELIGHT
Industry
FR
UTC+1
Peterka
Darcy
New York
Columbia University
Univ.
US
UTC+5
Piazza
Simonluca
Genoa
Genoa Instruments
Industry
IT
UTC+1
Pihan
German
Boston
BIDMC/HARVARD
Univ.
US
UTC+5
Plantard
Laure
Basel
Friedrich Miescher Institute for Biomedical Research
Private lab
CH
UTC+2
Podder
Santosh
Pune
IISER Pune
Gov.
IN
UTC-8
Politi
Antonio
Göttingen
Max Planck Institute of Biophysical Chemistry
MPG
DE
UTC+1
Prakash
Kirti
London
National Physical Laboratory
Gov.
GB
UTC+-0
Rad
Dorsa
Sydney
ANZAC Research Instittue
Univ.
AU
UTC+11
Rezgui
Rachid
Abu Dhabi
NYU Abu Dhabi
Univ.
AE
UTC+4
Ritz
Sandra
Mainz
Institute of Molecular Biology – IMB
Univ.
DE
UTC+2
Robson
Joanne
Durham
Durham University
Univ.
GB
UTC+-0
Saeed
Mezida
Edgabaston
University of Birmingham
Univ.
GB
UTC+-0
Saint-Hilaire
Cadisha
Paris
Curie Institute
Univ.
FR
UTC+1
Sanetrnik
Vojtech
Brno
Telight
Industry
CZ
UTC+1
Schmidt
Alfonso
Kelburn Parade
Malaghan Institute of Medical Reserach
Private lab
NZ
UTC+12
Schmied
Jürgen
Gräfelfing
GATTAquant GmbH
Industry
DE
UTC+2
Schreiner
Ryan
New York
Weill Cornell Medicine
Univ.
US
UTC-4
Schuchardt
Chris
Jena
Carl Zeiss Jena GmbH
Industry
DE
UTC+1
Schumann
Christian
Wetzlar
Leica Microsystems CMS GmbH
Industry
DE
UTC+1
Schwartz
Stanley
Austin
Nikon
Industry
US
UTC-5
Segal
Gabriela
Melbourne
University of Melbourne
Univ.
AU
UTC+10
Seitz
Arne
Lausanne
EPFL
Univ.
CH
UTC+2
Selbach
Florian
Gräfelfing
GATTAquant GmbH
Industry
DE
UTC+2
Shakouri
Mahsa
Heidelberg
DKFZ
Industry
DE
UTC+12
Shaw
Mike
London
National Physical Laboratory
Gov.
GB
UTC+1
Shemesh
Michal
Delft
TUD
Univ.
NL
UTC+1
Silván Alcaraz
Ágata
Copenhagen
University of Copenhagen
Univ.
DK
UTC+1
Simm
Alan
Liverpool
Liverpool John Moores University
Univ.
GB
UTC+-0
Smith
Carlas
Delft
Delft University of technology
Univ.
NL
UTC+2
Soubies
Emmanuel
Toulouse
CNRS
Univ.
FR
UTC+2
Sporbert
Anje
Berlin
GerBi-GMB WG 1
Helmholtz
DE
UTC+2
Srinivasan
Sathya
Beaverton
ONPRC
Univ.
US
UTC-8
Steinberger
Joseph
Rehovot
Weizmann Institute of Science
Univ.
IL
UTC+3
Stifani
Nicolas
Montréal
Université de Montréal
Univ.
CA
UTC-5
Su
Yingying
Riverwood
The University of Sydney
Univ.
AU
UTC+10
Tassis
Kostas
Leiden
Leiden University
Univ.
NL
UTC+1
Thomson
Darren
Exeter
University of Exeter
Univ.
GB
UTC+1
Thuenauer
Roland
Hamburg
Heinrich Pette Institute
Leibniz
DE
UTC+2
Titus
Steve
Rising SUn
ThermoFisher
Industry
US
UTC-5
Tzavaras
Nikos
New York
Icahn School of Medicine at Mount Sinai
Univ.
US
UTC-5
van der Voort
Hans
Hilversum
Scientific Volume Imaging BV
Industry
NL
UTC+1
Vendrell-Flotats
Meritxell
Cerdanyola del Vallès
Universitat Autònoma de Barcelona
Univ.
ES
UTC+1
Verma
Jitender
Sonepat
Ashoka University
Univ.
IN
UTC+5
Vetter
Tatyana
Columbus
Nationwide Children’s Hospital
Univ.
US
UTC-5
Vicidomini
Giuseppe
Genoa
Istituto Italiano di Tecnologia
Gov.
IT
UTC+1
Vogt
Michael
Aachen
IZKF Aachen RWTH University Hospital
Univ.
DE
UTC+1
Voort
Nicolaas
Hilversum
Scientific Volume Imaging BV
Industry
NL
UTC+2
Wee
Erika
Cold Spring Harbor
Cold Spring Harbor Laboratory
Univ.
US
UTC-5
Weiss
Kurt
Madison
University of Wisconsin-Madison
Univ.
US
UTC-6
Weiss
Pierre
Toulouse
CNRS
Univ.
FR
UTC+2
Yao
Jie
Seattle
Allen Institute
Private lab
US
UTC-7
Zhu
Chengxi
Cambridge
University of Cambridge
Univ.
GB
UTC+-0