WG 4 – System chromatic aberration and co-registration

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WG 4 – Objectives

Chromatic aberration refers to possible artifacts caused by the wavelength dependency of an imaging system’s optical properties, with the result that two colors arising from the same physical location within the sample appear separated in the image.  Such artifacts result from the optical design of the system (e.g., well-corrected versus poorly corrected objective lenses), the manufacturing tolerances of the system components, and the alignment of the optical components.

Co-registration accuracy more generally refers to the system’s ability to co-localize dyes of different wavelengths emitting from the same object within a particular experimental set-up. This can be affected by both the experimental set-up and the system architecture. Working within the assumption that microscope users are ultimately interested in co-registration accuracy, WG4 aims to use sub-resolution and larger multi-colored bead preparations to measure co-registration accuracy. Alternative tools for performing these measurements will also be evaluated. WG4 will compare reproducibility across different laboratories to determine the best protocol.

WG 4 – Co-Chairs

WG 4 - Latest News

We welcome our first member from the United Arab Emirates. Rachid Rezgui of NYU Abu Dhabi joined the Working Groups 1-7, 9, 10 and 13. Change the Quality of Light Microscopy Imaging as member of QUAREP-LiMi join us here.

We welcome our first member from the China. Xiaoming Li from ShanghaiTech University,  joined the Working Groups 1 to 11. Change the Quality of Light Microscopy Imaging as member of QUAREP-LiMi join us here.

 Last meeting: 17th June 2025, 5:00 pm

48th meeting, 17h of June 2025

Meeting date

17/06/2025 @ 5:00 PM (CET/CEST)

Meeting summary

LacJ presented a report from the last SC meeting. AzeM informed the group that a new OB representative is needed. The group discussed the recent modifications to the protocol made by DauA and agreed on the next steps to finalize it: DauA and AzeM will collaborate on developing the discussion section. It was also decided to begin acquiring new datasets using widefield (WF) and spinning disk confocal (SDC) systems. A new batch of slides containing 1 µm TetraSpeck beads will be sent to those interested in acquiring images. The group will take a summer break in July and August, with the next meeting scheduled for September 16th.

47th meeting, 20th of May 2025

Meeting date

20/05/2025 @ 5:00 PM (CET/CEST)

Meeting documentation

46th meeting, 15th of April 2025

Meeting date

15/04/2025 @ 5:00 PM (CET/CEST)

Meeting summary

WG04 members began the meeting by discussing feedback from the annual meeting. The author list on protocols.io was also addressed, and it was agreed that all co-authors should enter their full institutional address in the affiliation section under the “Organization” sub-tab. The group also discussed the upload of WG04 data into OMERO and reviewed the adopted naming conventions. Regarding next steps, members agreed to move forward with improving the first version of the WG04 protocol. The immediate goal is to adapt the protocol for use on confocal spinning disk and widefield microscopy systems.

Meeting documentation

45th meeting, 18th of March 2025

Meeting date

13/03/2025 @ 5:00 PM (CET/CEST)

Meeting documentation

44th meeting, 18th of February 2025

Meeting date

18/02/2025 @ 5:00 PM (CET/CEST)

Meeting summary

In the latest WG04 meeting, members reviewed the feedback received from a co-chair of another WG on the first version of the protocol. Final adjustments were discussed and agreed upon to refine the document before publication. Additionally, the group addressed authorship matters, ensuring proper acknowledgment of contributions and clarifying the necessary updates to affiliations on protocols.io.

Meeting documentation

43rd meeting, 21st of January 2025

Meeting date

21/01/2025 @ 5:00 PM (CET/CEST)

Meeting documentation

42nd meeting, 17th of December 2024

Meeting date

17/12/2024 @ 5:00 PM (CET/CEST)

Meeting documentation

41st meeting, 19th of November

Meeting date

19/11/2024 @ 5:00 PM (CET/CEST)

Meeting summary

LacJ shared the key points discussed during the SC meeting on 2024/11/12. The remainder of the meeting was dedicated to discussing the outline and content of the protocol.

Meeting documentation

40th meeting, 15th of October 2024

Meeting date

15/10/2024 @ 5:00 PM (CET/CEST)

Meeting summary

LacJ shared the key points discussed during the SC meeting on 2024/10/01 and presented the work on the data model to the WG members. The group focused on discussing the organization and content of the protocol, and deadlines were set to ensure its finalization. Our next WG meeting will be on November 19th.

Meeting documentation

39th meeting, 17th of September 2024

Meeting date

17/09/2024 @ 5:00 PM (CET/CEST)

Meeting documentation

Memberlist WG 4

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