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WG 5 – Lateral and axial resolution

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WG 5 – Objectives

This WG focuses on the microscope lateral and axial resolution, which is essential for reporting size measurements of near- resolution limit objects or distances between them. Resolution is highly related to the objective quality but depends strongly upon other parameters ranging from the sample preparation to the signal detection.

The WG aims to define sample preparation, image acquisition, and data analysis protocols for testing resolution, first using sub-resolution fluorescent bead preparations and second employing alternative pattern-based methods. Monitoring the resolution (Point Spread Function in the case of beads) over time will identify possible aberrations in the system. Pooling the data from multiple laboratories within the WG will allow them to compare reproducibility for sample preparation, data acquisition, and data analysis tools, thereby determining a robust, easy-to-use protocol to propose to the community.

WG 5 – Co-Chairs

WG 5 - Latest News

First protocol for Monitoring the point spread function

The WG 5 released its first protocol “Monitoring the point spread function for quality control of confocal microscopes“. The protocol is featured on

We welcome our first member from Turkey

We welcome our first member from Turkey.  Salim Can Akçınar of Ege University joined the Working Groups 5,6 and 12. Change the Quality of Light Microscopy Imaging as member of QUAREP-LiMi join us here.

We welcome our first member from the United Arab Emirates

We welcome our first member from the United Arab Emirates. Rachid Rezgui of NYU Abu Dhabi joined the Working Groups 1-7, 9, 10 and 13. Change the Quality of Light Microscopy Imaging as member of QUAREP-LiMi join us here.

 

Last meeting: 9th April 2026, 3:30 pm

Release of protocol “Monitoring the point spread function for quality control of confocal microscopes“.

48th meeting, 14th of April 2025

Meeting date

14/04/2025 @ 3:30 PM (CET/CEST)

Meeting summary

The protocol V2 was discussed and it was voted to pass the next version of the protocol to the SC. For the upcoming ELMI workshop, plans were initiated to host a one-hour session, considering point source and line patterns, and coordinating tasks among participants. An update on the paper shared insights on confocal SBR and r² values, noting differences due to noise factors. Discussions included data analysis techniques and the challenges of data variance over time. The meeting concluded with a call for organizing data in OMERO and an update on a new ISO norm for metadata, inviting community participation. The next meeting is postponed by a week to accommodate a French national holiday.

Meeting documentation

47th meeting, 13th of March 2025

Meeting date

13/03/2025 @ 3:30 PM (CET/CEST)

Meeting documentation

46th meeting, 13th of February 2025

Meeting date

13/02/2025 @ 3:30 PM (CET/CEST)

Meeting documentation

45th meeting, 9th of January 2025

Meeting date

09/01/2025 @ 3:30 PM (CET/CEST)

Meeting documentation

44th meeting, 14th of November 2024

Meeting date

14/11/2024 @ 3:30 PM (CET/CEST)

Meeting documentation

43rd meeting, 10th of October 2024

Meeting date

10/10/2024 @ 3:30 PM (CET/CEST)

Meeting documentation

42nd meeting, 19th of September 2024

Meeting date

14/09/2024 @ 3:30 PM (CET/CEST)

Meeting documentation

41st meeting, 11th of July 2024

Meeting date

11/07/2024 @ 3:30 PM (CET/CEST)

Meeting documentation

40th meeting, 13th of June 2024

Meeting date

13/06/2024 @ 3:30 PM (CET/CEST)

Meeting summary

We discussed the ELMI workshop, including how it went and what we could improve. We also covered the process of OMERO uploads and linking them to Airtable for tracking results, as well as the filenaming nomenclature.

Meeting documentation

39th meeting, 24th of May 2024

Meeting date

24/05/2024 @ 3:30 PM (CET/CEST)

Meeting documentation

Memberlist WG 5