WG 14 – Room Environment

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WG 14 – Objectives

A stable environment is crucial to operate state of the art microscopes. Commercial instruments are designed to operate under specific room environment parameters, including temperature, humidity, vibration, dust, and electrical/electromagnetic or acoustical noise. 

It is important to adhere to these specifications to ensure accurate and reproducible results. Currently, it is unclear for the light microscopy community how to accurately and routinely measure these parameters and relate own measurements with the required specifications. 

WG14 aims to establish measurement protocols for light microscopy room requirements. Our initial goal will be to develop protocols for measuring temperature at key locations in the room and at the microscope.

WG 14 – Co-Chairs

WG 14 - Latest News

 Upcoming meeting: 19th August 2026, 5:00 pm
Last meeting: 15th July 2026, 5:00 pm

22nd meeting, 19th of August 2026

Meeting date

19/08/2026 @ 5:00 PM (CET/CEST)

Meeting summary

Preliminary Agenda:

  1. Organisational matters and News
  2. Working on our Protocol Draft
  3. Next steps

21st meeting, 15th of July 2026

Meeting date

15/07/2026 @ 5:00 PM (CET/CEST)

Meeting summary

Agenda:

  1. Organisational matters and News
  2. Working on our Protocol Draft
  3. Next steps

Summary:

The Working Group 14 meeting focused on progress updates for their room temperature measurement protocol draft, with new member Jakub joining as validation lead. The group discussed challenges with Aim 3 of the protocol, which involves analysing temperature gradients over time periods of 1 hour, 12 hours, and 24 hours using multiple sensors per room. They agreed that two sensors would be sufficient for Aim 2 (equipment requirement check) and at least three sensors would be required for Aim 3 (room behaviour determination). The team assigned homework tasks including data analysis using the formulas from the protocol draft, purchasing metrology sensors from ELV for measurements, and contacting equipment vendors to gather updated room temperature requirement specifications. They also planned to add a new appendix discussing how room temperature fluctuations affect incubation systems and their stability.

20th meeting, 20th of May 2026

Meeting date

20/05/2026 @ 5:00 PM (CET/CEST)

Meeting summary

Agenda:

  1. Organisational matters and News
  2. Working on our Protocol Draft
  3. Next steps

Summary:

After discussing organisational matters, we have been working on our protocol draft. We agreed that to have more reliable numbers published within step 4 ff, we need to measure several rooms in different institutes. Our aim would be to measure in several institutes together 6-10 rooms. We discussed the analysis part for aim 3 and came to the conclusion that for developing a clear idea what could be needed for a more detailed room temperature description (aim 3) we should collect scenarios, setups, and maybe even first measurements which could showcase the need for aim 3 measurements and thereby suggest a particular analysis workflow.

19th meeting, 15th of April 2026

Meeting date

15/04/2026 @ 5:00 PM (CET/CEST)

Meeting summary

Agenda:

  1. Organisational matters and News
  2. Discuss meaning of given temparature variations for light microscopy
  3. Working on our Protocol Draft
  4. Next steps

Summary:

After organizational matters and a report from the SC meeting, we agreed on the meaning of “mean room temperature” and the +/- values. The given temperature is the set temperature, the AC should reach. Thus, when your measured mean temperature is too far off from the set temperature, something is also wrong. The +/- values are measured around the reached mean value. Finally, we have been working on our protocol draft step 4 and ff.

18th meeting, 18th of February 2026

Meeting date

18/02/2026 @ 5:00 PM (CET/CEST)

Meeting summary

Agenda:
  1. Organisational matters and News
  2. Discuss meaning of given temparature variations for light microscopy
  3. Working on our Protocol Draft
  4. Next steps
  Meeting Summary: After organizational matters and a report from the SC meeting (Quarep will have a community room at ELMI2026 in Coimbra, but it will be too early to give a WG14 workshop in there), a quick update on the dexel-pixel definitions as well as the LiMi-model was given. We discussed the meaning of “mean room temperature” and the +/- values. We should include examples in the protocol. We have been working on our protocol draft step 4 and ff. We are still not sure about temperature gradients between multiple sensors in the room and how big gradients can be accepted: Maybe a mean delta T of 1.5°C +/-SD? We should measure it and determine this in about 6 to 10 rooms to have a more reliable value. Next, we want to prepare text suggestion for step 4 aim 3. We need to discuss and start to measurements with 3 sensors in a room according to protocol aim 2. We will enquire about whether we can buy 1-2 sets of 3 sensors for QUAREP to send around.

17th meeting, 21st of January 2026

Meeting date

21/01/2026 @ 5:00 PM (CET/CEST)

Meeting summary

Agenda:
  1. Organisational matters and News
  2. Working on our Protocol Draft
  3. Next steps
Summary:
We started the meeting with a short report from the January steering committee meeting. As a result, we agreed to not plan a workshop at ELMI 2026, but rather aim for doing it for ELMI 2027.
We continued the meeting with our main agenda point, working on our protocol draft. In particular, we worked on finishing the comment for Aim2 in step4 of the protocol.
During the discussion, we focused on temperature analysis requirements and specific temperature variation limits for different room conditions.
In addition, we discussed sensor placement and temperature measurement requirements for microscope rooms, with particular attention to temperature gradients and fluctuations that could impact imaging quality.

16th meeting, 19th of November 2025

Meeting date

19/11/2025 @ 5:00 PM (CET/CEST)

Meeting summary

Agenda:
  1. Organisational matters and News
  2. Working on our Protocol Draft
  3. Next steps

15th meeting, 15th of October 2025

Meeting date

15/10/2025 @ 5:00 PM (CET/CEST)

Meeting summary

Agenda:
  1. Organisational matters and News
  2. New name for NBO-Q model
  3. Working on our Protocol Draft
  4. Next steps

14th meeting, 20th of August 2025

Meeting date

20/08/2025 @ 5:00 PM (CET/CEST)

Meeting summary

Agenda:
  1. Organisational matters and News
  2. Working on our Protocol Draft
  3. Next steps
 
At the beginning of our meeting we confirmed to skip our September meeting and reconvene in October. This was followed by a report from the last steering committee meeting. The SC addressed protocol implementation and funding opportunities, including discussions about a potential grant. We then continued to work on our protocol draft. In particular, we focused on temperature monitoring requirements for different microscope systems, including sensor placement and measurement methods. Mainly, we worked on step 4. We discussed which and how many sliding windows for temperature measurement and evaluation is needed with respect to our three aims, and how many sensors are needed for the three different aims.
Homework has been distributed to prepare for the next meeting, which will take place on October 15th 2025.

13th meeting, 18th of June 2025

Meeting date

18/06/2025 @ 5:00 PM (CET/CEST)

Meeting summary

Agenda:
  1. Organisational matters and News
  2. Report from ELMI 2025
  3. Working on our Protocol Draft
  4. Next steps

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