WG 14 – Objectives
A stable environment is crucial to operate state of the art microscopes. Commercial instruments are designed to operate under specific room environment parameters, including temperature, humidity, vibration, dust, and electrical/electromagnetic or acoustical noise.
It is important to adhere to these specifications to ensure accurate and reproducible results. Currently, it is unclear for the light microscopy community how to accurately and routinely measure these parameters and relate own measurements with the required specifications.
WG14 aims to establish measurement protocols for light microscopy room requirements. Our initial goal will be to develop protocols for measuring temperature at key locations in the room and at the microscope.
WG 14 – Co-Chairs
Call:
+49-351-210-2522
Email:
schroth@mpi-cbg.de
Britta Schroth-Diez
Call:
+49 228 43302-894
Email:
hans.fried@dzne.de
Hans-Ulrich Fried
WG 14 - Latest News
Upcoming meeting: 19th August 2026, 5:00 pm
Last meeting: 15th July 2026, 5:00 pm

22nd meeting, 19th of August 2026
Meeting date
Meeting summary
Preliminary Agenda:
- Organisational matters and News
- Working on our Protocol Draft
- Next steps

21st meeting, 15th of July 2026
Meeting date
Meeting summary
Agenda:
- Organisational matters and News
- Working on our Protocol Draft
- Next steps
Summary:
The Working Group 14 meeting focused on progress updates for their room temperature measurement protocol draft, with new member Jakub joining as validation lead. The group discussed challenges with Aim 3 of the protocol, which involves analysing temperature gradients over time periods of 1 hour, 12 hours, and 24 hours using multiple sensors per room. They agreed that two sensors would be sufficient for Aim 2 (equipment requirement check) and at least three sensors would be required for Aim 3 (room behaviour determination). The team assigned homework tasks including data analysis using the formulas from the protocol draft, purchasing metrology sensors from ELV for measurements, and contacting equipment vendors to gather updated room temperature requirement specifications. They also planned to add a new appendix discussing how room temperature fluctuations affect incubation systems and their stability.

20th meeting, 20th of May 2026
Meeting date
Meeting summary
Agenda:
- Organisational matters and News
- Working on our Protocol Draft
- Next steps
Summary:
After discussing organisational matters, we have been working on our protocol draft. We agreed that to have more reliable numbers published within step 4 ff, we need to measure several rooms in different institutes. Our aim would be to measure in several institutes together 6-10 rooms. We discussed the analysis part for aim 3 and came to the conclusion that for developing a clear idea what could be needed for a more detailed room temperature description (aim 3) we should collect scenarios, setups, and maybe even first measurements which could showcase the need for aim 3 measurements and thereby suggest a particular analysis workflow.
19th meeting, 15th of April 2026
Meeting date
Meeting summary
Agenda:
- Organisational matters and News
- Discuss meaning of given temparature variations for light microscopy
- Working on our Protocol Draft
- Next steps
Summary:

18th meeting, 18th of February 2026
Meeting date
Meeting summary
- Organisational matters and News
- Discuss meaning of given temparature variations for light microscopy
- Working on our Protocol Draft
- Next steps

17th meeting, 21st of January 2026
Meeting date
Meeting summary
- Organisational matters and News
- Working on our Protocol Draft
- Next steps

16th meeting, 19th of November 2025
Meeting date
Meeting summary
- Organisational matters and News
- Working on our Protocol Draft
- Next steps

15th meeting, 15th of October 2025
Meeting date
Meeting summary
- Organisational matters and News
- New name for NBO-Q model
- Working on our Protocol Draft
- Next steps

14th meeting, 20th of August 2025
Meeting date
Meeting summary
- Organisational matters and News
- Working on our Protocol Draft
- Next steps

13th meeting, 18th of June 2025
Meeting date
Meeting summary
- Organisational matters and News
- Report from ELMI 2025
- Working on our Protocol Draft
- Next steps
Memberlist WG 14
| Last name | First Name | Town | Organisation Affiliation | Lab Affiliation | Country | Time Zone |
|---|---|---|---|---|---|---|
| Aleksandrova | Nataliia | Freiburg im Breisgau | UNI Freiburg | Univ. | DE | UTC+1 |
| Alexopoulos | Ioannis | Giessen | ILH/CIGL Multiscale Imaging Platform | Univ. | DE | UTC+2 |
| Ali | Rizwan | Riyadh | King Abdullah International Medical Research Center | Univ. | SA | UTC+3 |
| Alshafie | Walaa | Ashburn | Janelia research campus | Univ. | US | UTC-4 |
| Aulner | Nathalie | Paris | Institut Pasteur | Private lab | FR | UTC+2 |
| Corcoran | David | Coventry | University of Warwick | Univ. | GB | UTC+-0 |
| DELNERY | Elaine | Paris | Global-BioImaging | Univ. | FR | UTC+2 |
| Doherty | Glen | New York | Icahn School of Medicine at Mount Sinai | Univ. | US | UTC-4 |
| Fazeli | Elnaz | Helsinki | University of Turku / University of Helsinki | Univ. | FI | UTC+1 |
| Fried | Hans | Bonn | GerBi-GMB WG 1 | Helmholtz | DE | UTC+2 |
| Gewerth | Jacob | Roseville | Maka Solutions LLC | Industry | US | UTC-7 |
| Goda | Kazuhito | Hachioji | EVIDENT | Industry | JP | UTC+9 |
| Goetsch | Miki | Fujinomiya | Tokai Hit Co., Ltd. | Industry | JP | UTC+9 |
| Grunwald | David | Worcester | University of Massachusetts | Univ. | US | UTC-4 |
| Hu | Jianjiang | Huddinge | Karolinska Institutet | Univ. | SE | UTC+1 |
| Kaiping | Arnica | Freiburg | University of Freiburg | Univ. | DE | UTC+2 |
| Köning | Rainer | Braunschweig | PTB Braunschweig | Gov. | DE | UTC+1 |
| Kukat | Christian | Cologne | GerBi-GMB WG 1 | MPG | DE | UTC+2 |
| Lai | Pok Yui | Oxford | University of Oxford | Univ. | GB | UTC+1 |
| Lanzaro | Luca | Pozzuoli (NA) | OKOLAB | Industry | IT | UTC+2 |
| Larsen | DeLaine | Hayward | Eikon Therapeutics | Industry | US | UTC-8 |
| Liljeström | Mikko | Helsinki | University of Helsinki | Univ. | FI | UTC+2 |
| Liu | Tristan | New York | Columbia University | Univ. | US | UTC-4 |
| Machovina | Tyler | Sewickley | Okolab | Industry | US | UTC+5 |
| Monterroso | Baptiste | Nice | University of Nice | Univ. | FR | UTC+2 |
| Nitschke | Roland | Freiburg | University of Freiburg, Life Imaging Center | Univ. | DE | UTC+2 |
| Parslow | Adam | Melbourne | Baker Heart and Diabetes Institute | Private lab | AU | UTC+10 |
| Pospíšil | Jakub | Dresden | QUAREP-LiMi / Technische Universität Dresden | Univ. | DE | UTC+1 |
| Schroth-Diez | Britta | Dresden | MPI-CBG | MPG | DE | UTC+2 |
| Silván Alcaraz | Ágata | Copenhagen | University of Copenhagen | Univ. | DK | UTC+1 |
| Srinivasan | Sathya | Beaverton | ONPRC | Univ. | US | UTC-8 |
| Tsuchiya | Takanori | Fujinomiya | TOKAI HIT | Industry | JP | UTC+9 |
| Węgierski | Tomasz | Warsaw | International Institute of Molecular and Cell Biology | Gov. | PL | UTC+2 |