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WG 3 – Field Homogeneity

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WG 3 – Objectives

Illumination field uniformity is critical for quantitative imaging when comparing fluorescence intensities across a field-of-view (FOV) or a large tile of images capturing an entire sample. If the illumination is not constant over a large area, the fluorescence intensities will not represent the inherent fluorescence but rather the location within the image. Thus, WG3 aims at defining a set of universal protocols to assess the uniformity of illumination (i.e., “flatness” of field) over the FOV of any photon-based imaging system and allow for correction of any non-uniformity. These protocols will identify the necessary tools, the procedures required to perform the measurements, and the analysis methods required for their interpretation. WG3 will also define criteria regarding the cut-off for acceptability and the need for correction. A database will be created with ideal images of uniform fields-of-view from different microscope modalities and settings to be used as a reference by the community and validate the protocol and criteria.

WG 3 – Co-Chairs

WG 3 - Latest News

We welcome our first member from China

We welcome our first member from the China. Xiaoming Li from ShanghaiTech University,  joined the Working Groups 1 to 11. Change the Quality of Light Microscopy Imaging as member of QUAREP-LiMi join us here.

We welcome our first member from Russia

We welcome the first member from the Russia. Maxim Petrov from SIAMS Ltd,  joined the Working Groups 2, 3 and 10. Change the Quality of Light Microscopy Imaging as member of QUAREP-LiMi join us here.

 Last meeting: 12th March 2026, 5:00 pm

48th meeting, 12th of March 2026

Meeting date

12/03/2026 @ 5:00 PM (CET/CEST)

Meeting documentation

47th meeting, 12th of February 2026

Meeting date

12/02/2026 @ 5:00 PM (CET/CEST)

Meeting documentation

46th meeting, 8th of January 2026

Meeting date

08/01/2026 @ 5:00 PM (CET/CEST)

Meeting documentation

45th meeting, 9th of October 2025

Meeting date

09/10/2025 @ 5:00 PM (CET/CEST)

Meeting documentation

44th meeting, 11th of Septemeber 2025

Meeting date

11/09/2025 @ 5:00 PM (CET/CEST)

Meeting documentation

43rd meeting, 24th of July 2025

Meeting date

24/07/2025 @ 5:00 PM (CET/CEST)

Meeting documentation

42nd meeting, 12th of June 2025

Meeting date

12/06/2025 @ 5:00 PM (CET/CEST)

Meeting documentation

41st meeting, 8th of May 2025

Meeting date

08/05/2025 @ 5:00 PM (CET/CEST)

Meeting documentation

40th meeting, 13th of March 2025

Meeting date

13/03/2025 @ 5:00 PM (CET/CEST)

Meeting documentation

39th meeting, 13th of February 2025

Meeting date

13/02/2025 @ 5:00 PM (CET/CEST)

Meeting documentation

Memberlist WG 3