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Working Group 1

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11th Meeting, 1st of September 2021

Meeting summary

We spent the meeting reviewing the edits and comments everyone made in the first two pages of the main document. Amongst other things, we discussed adding more specificity to the goal of this protocol in the introduction, proposed to emphasize Laser Safety remarks with bold text, references and symbols, to add a CAD file of Roland’s holder, and write system specific instructions to the appendix section. A Zotero group library has been created and link sent to all (please communicate with Nathalie if you need help including a reference).

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10th meeting, 2nd of June 2021

Meeting summary

We reviewed comments in the main document, discussing comments and suggestions and adding tips on safety, environmental conditions, and type of laser used. We also discussed the potential damages a laser can cause to an objective, the difference in laser power measurements obtained from different laser power meter brands/sensors and the effect of temperature on your measurements. The subgroups “Automation” and “Powermeter” reported briefly their progress.

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9th meeting, 5th of May 2021

Meeting summary

We reviewed comments in the main document, discussed discrepancy between measurements made with different power meter models on the same microscope and same laser, commented different graphs to display all measurements made by the group in their respective labs so far, to help define what a “stable” behavior is. We decided to set up a Zotero shared library for reference articles. The automation sub-group presented the project of a GitHub repository for scripts.

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8th meeting, 7th of April 2021

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7th meeting, 3rd of March 2021

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6th meeting, 3rd of February 2020

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Working Group 2

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11th meeting, 13th of September 2021

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10th meeting, 12th of July 2021

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9th meeting, 14th of June 2021

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8th meeting, 10th of May 2021

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7th meeting, 12th of April 2021

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6th meeting, 8th of March 2021

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Working Group 3

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4th meeting, 6th of May 2021

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3rd meeting, 1st of April 2021

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2nd meeting, 2nd of February 2021

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1st meeting, 6th of October 2020

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Working Group 4

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9th meeting, 21st of September 2021

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8th meeting, 20th of July 2021

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7th meeting, 15th of June 2021

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6th meeting, 18th of May 2021

Meeting summary

We have generated a detailed protocol and are currently testing the following parameters: 1) how does bead size affect the coregistration accuracy. We test 200nm, 1µm, and 5µm beads at the moment. 2) are the suggested settings from the iso manual neccessary, or can we generate a more convenient protocol. We test the impact of pixel size and z-step size (16nm/40nm, and 70/200nm). From first measurements, we have seen that a variation from speciment preparation can be rather high. Thus, one of us (Aurelien) is creating the samples for the rest of WG4.  

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5th meeting, 20th of April 2021

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4th meeting, 16th of March 2021

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Working Group 5

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11th meeting, 9th of September 2021

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10th meeting, 8th of July 2021

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9th meeting, 10th of June 2021

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8th meeting, 6th of May 2021

Meeting summary

WG 5 have principally been testing beads for PSF measurements, using different formats (polystyrene, DNA origami, nanodots, printed patterns). We have identified an optimal size range for confocal and widefield imaging, and a re still testing the ease of use, functionality and robustness of several of these formats. We have begun a draft methodology which we propose to publish for Confocal PSF measurement using beads before developing the method further for other modalities, as well as investigating alternative methods, such as mirrors for axial resolution tests.

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7th meeting, 11th of March 2021

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6th meeting, 11th of February 2021

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Working Group 6

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6th meeting, 8th of September

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4th meeting, 12th of May 2021

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3rd meeting, 13th of April 2021

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2nd meeting, 10th of March 2021

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1st meeting, 10th of February 2021

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Working Group 7

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8th meeting, 20th of September

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7th meeting, 19th of July 2021

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6th meeting, 17th of May 2021

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5th meeting, 19th of April 2021

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4th meeting, 15th of March 2021

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3rd meeting, 11th of November 2020

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Working Group 8

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10th meeting, 7th of January 2021

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9th meeting, 21st of December 2020

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8th meeting, 14th of December 2020

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7th meeting, 10 of December 2020

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6th meeting, 1st of December 2020

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5th meeting, 19th of November 2020

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Working Group 9

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8th meeting, 7th of September

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7th meeting, 13th of July 2021

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6th meeting, 1st of June 2021

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5th meeting, 4th of May 2021

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4th meeting, 6th of April 2021

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3rd meeting, 2nd of February 2021

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Working Group 10

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11th meeting, 14th of September 2021

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10th meeting, 8th of June 2021

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9th meeting, 25th of May 2021

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8th meeting, 11th of May 2021

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7th meeting, 13th of April 2021

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6th meeting, 23rd of March 2021

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Working Group 11

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6th meeting, 23rd of September 2021

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5th meeting, 21st of July 2021

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4th meeting, 27th of May

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3rd meeting, 25th of March 2021

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2nd meeting, 24th of February 2021

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1st meeting, 28th of January 2021

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