WG 2 – Detection system performance

1) First we discussed some general QUAREP-LiMi news:

– report from the WG9 & co-chairs meeting on May 2nd (feedback from general meeting, QUAREP-LiMi Organigram)

2) Second we talked about the preparation of our contribution for ELMI 2023:

– presenters, preparation support team members, dates for preparation meetings, abstract was sent, date for the actual workshop, which setups will be available

3)  Third, we review the annual meeting:

– intro session: needs to be improved, idea from WG9: have meeting schemes (Spring: external & outreach, Fall: internal – update about work group progress

– our break out room: comments to follow up (advice about when a detector is OK / not OK; make protocol available)

– main panel (great) & general feedback (general goal of QUAREP needs to be made more clear)

– our survey (short look at the results, need to be looked at closer later)

4)  Mathias reported from his EMVA1288 training (will optimize and upload the slides):

– report followed by discussion about how to use the material, how to adapt it to our LiMi needs, we always use EMVA1288 as guidance

5)  Last we postponed David’s report again, to the June meeting and agreed on the next steps.

Draft versions of the workgroup presentation and survey for the General assembly meeting were shared and suggestions for improvements were given. Discussion topics for the breakout session were given. Mathias’ report from his EMVA1288 training and the update on David MacFadden’s analysis script will be discussed during the meeting in May.

1. First we discussed some general QUAREP-LiMi news:
   • QUAREP-LiMi is now a community partner of image.sc, we potentially need to contribute to the moderating in the future
   • there is a community room at ELMI for QUAREP and other imaging communities to do 6 workshops, WG2 member contribution is welcome
   • the French MetrologyJ-QC group is very interested in a collaboration with us in WG2, we will invite them to a future WG2 meeting
   • WG12 paper is in the review process of Nature methods
2. Second we collected ideas on which topics to discuss in the breakout room. It was agreed to especially include open questions and problems: challenge how to measure sensitivity; our strategy of community roll-out; current problems we are facing: UUIDs; data handling, storage, and retrieval. We also collected some potential survey questions. A small team was formed to prepare the breakout room, the short introduction video and questions for a possible survey; all of which will be presented during our next WG2 meeting.
3. Third followed the reports from the small group measurement teams: for sample preparation, we now have the text for the marker-pen sample on protocols.io (please check it out). A text is planned for the Chromaslide ramp. MathiasH went to the EMVA1288 certification course. He will report in our next meeting.

We closed the meeting by agreeing on the next steps.

After first talking about organisational news, we discussed the changes in date and plans for our upcoming QUAREP-LimI general assembly meeting. It is going to be held online on April 26th 2023 3-6PM. We decided that we want to participate with our own breakout room and formed a small preparation team which will also think about potential questions for a survey. A short video presentation needs to be prepared as well. Next the small measurement teams reported their results. Team CET focussed on test samples and found the marker pen as bright enough, easy to prepare and use. Team CET2 focused on the protocol and its usability via protocols.io. There is still a lot of optimization to do (changing the order of the steps, optimizing the run behavior of the protocol). This discussion was followed by the report from our training session with Emma Ganley from the protocols.io team. At the end of the meeting we summarized the main next steps: simplify our protocols and the protocols structure, and updated our detection system behavior measurement excel sheet. The APE measurement reporting session is planned for our June meeting.

We used the opportunity of this being the first meeting in 2023 to look back at our achievements, but also challenges, last year. As a main challenge we identified the motivation and organisation for collecting data and analysing it. This is essential to test and optimise our protocols to be able to finalise and subsequently publish them. We therefore decided to form small groups of 3-4 people doing the measurements together. In addition, we discussed to have our aims and next steps smaller and more tight, focus on one aim only. This way we will steadily move forward to eventually reach our goal. Our protocols group gave a brief update on the development of our WG2 protocols in the protocols.io QUAREP-LiMi workspace and informed about the planned training session with Emma Ganley from the protocols.io team. At the end of the meeting we summarised the main next steps: do measurements in the small groups and contact the protocols.io support team about our needs.

We started with a brief report from WG9&co-chairs last meeting, focusing mainly on the next general assembly meeting of QUAREP-LiMi. In this meeting, we then had a big discussion on Metadata. There is a specific need of finding a good way to collect, name and recover image files.Try to find an agreement on naming the file isn’t universally acceptable. Therefore, the idea is to find a way to recover the most important metadata on acquisition parameters and device settings in a unique format. Omero could be the answer and it requires a list of metadata. In the same direction is also the request from Nature protocol and other editors for publication.