WG 2 – Detection system performance

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WG 2 – Objectives

WG2 focuses on the detection system, comprising the detection path and its detector(s), and how it measures the signal from the sample.

Members of WG2 aim to standardize the characterization of the detection system performance and create standard procedures for monitoring it over time, thereby revealing performance issues that could affect data reproducibility. Therefore, WG2 will define universal, externally measurable parameters applicable to any type of detector (e.g., photons, linearity, noise), together with measurement tools and protocols for measuring these parameters from common detector types. These universal parameters will be specified according to each distinct type of detector’s internal parameters, which have already been defined by the community. They will enable the evaluation and comparison of different detection systems, thus pinpointing the most suitable technology for given applications.

WG 2 – Co-Chairs

WG 2 - Latest News

We welcome the first member from the Russia. Maxim Petrov from SIAMS Ltd,  joined the Working Groups 2, 3 and 10. Change the Quality of Light Microscopy Imaging as member of QUAREP-LiMi join us here.

 

Upcoming meeting: 9th February 2026, 5:00 pm
Last meeting: 12th January 2026, 5:00 pm

  1. Subgroup: Area detector
    • Aims: Establish protocols for characterization of detection systems with an area detector.
    • Co-leads: Britta Schroth, David McFadden
    • Contact information: schroth@mpi-cbg.de
    • On-going tasks: 
      • Move measurement protocol to protocols.io
      • Test measurement protocol with marker pen as sample
      • Evaluate measurement and analysis parameters written down in Airtable
    • Cloud storage location (can be accessed as a member) → here
  2. Subgroup: Point detector
    • Aims: Establish protocols for characterization of detection systems with a point detector.
    • Co-leads: Gert-Jan Bakker, Andre Zeug
    • Contact information: gert-jan.bakker@radboudumc.nl
    • On-going tasks: 
      • Insert measurement protocol to protocols.io
      • Generate comments and hints for protocol
      • Evaluate measurement and analysis parameters written down in Airtable
    • Cloud storage location (can be accessed as a member) → here
  3. Subgroup: Test sample
    • Aims: investigate and define standardized samples for characterization of detection systems.
    • Co-leads: Valeria Berno, David McFadden
    • Contact information: berno.valeria@hsr.it
    • On-going tasks: 
      • Test and characterize standardized light sources for characterization of microscope’s detection systems.
    • Cloud storage location (can be accessed as a member) → here
  4.  

39th meeting, 11th of March 2024

Meeting date

11/05/2024 @ 5:00 PM (CET/CEST)

Meeting summary

Part of the meeting was planned to be the announcement of the results for our election of our WG2 co-chairs and representatives to the new governing bodies of QUAREP-LiMi (ERB, OB, SC). Unfortunately, we had some difficulties with the online voting platform. Therefore, we decided to do the elections via acclamation during the meeting. Results of the elections are: Co-chairs (for the next 2 years term): Gert-Jan Bakker, Valeria Berno, David McFadden, and Britta Schroth-Diez. ERB representative: David Grunwald, OB representative: Gerhard Holst. SC representative election will take place in our April meeting. The main topic of our 39th meeting was to discuss our potential contribution to the ELMI2024 community space. We agreed to do a similar workshop as we did last year, with particularly focusing on automation of the workflow and presentation of our protocols. To be able to do so, we need to finalise and ideally publish our protocols until ELMI2024 in June. To be able to do so, we formed two smaller groups to go over the protocols again until the April meeting. In addition to our protocols efforts, we aim to also finalise the joined WG2-7camera glossary project and keep working on our first paper.

Meeting documentation

38th meeting, 12th of February 2024

Meeting date

12/02/2024 @ 5:00 PM (CET/CEST)

Meeting summary

The main topic of the meeting was the presentation of the by-law initiative by David Grundwald. You can find the current versions of the by-law documents and the slide deck of the by-law presentation in our 38th meeting folder. The by-laws and their approval will also be the main topic of our QUAREP-LiMi general assembly meeting on April 16th 2024.
In addition to this important topic we also discussed our potential contribution to the community space during ELMI 2024. We agreed to discuss this in more detail during our March meeting.
Gert-Jan gave a literature pitch about a very interesting Mulit-photon microsocpy QC paper published recently in bioRxiv. His presentation can be found in our 38th meeting folder as well,
In addition, it was reported that the first version of the camera model and glossary is finished. It will be revisited and discussed by the camera model revision stakeholder group before it will be presented as a suggestion for model change to the whole QUAREP-LiMi consortium. The meeting finished with a short summary and reminder about the work to be done towards our paper.

Meeting documentation

37th meeting, 11th of December 2023

Meeting date

11/12/2023 @ 5:00 PM (CET/CEST)

Meeting summary

In short, the main topic of this meeting was to discuss a potential collaboration with the MetrologyJ_QC group. For that, Orestis and Julien joined us as guests.

We found that both our efforts are complementary and thus can nicely be combined. The MetrologyJ_QC analysis tool focusses on calculating camera read noise, DSNU (dark signal non-uniformity), and hot and cold pixels. Our WG2 analysis tool focusses on calculating the photon-conversion factor. In addition, we as WG2 aim to combine our analysis with an easy to use (automated) acquisition protocol.

Generally, QUAREP-LiMi needs to develop a nice overarching framework for running all our quality control scripts automatically together easily in one tool. Someone needs to take the lead for this endeavour. There is already great groundwork being done, thanks to Kees, for WG1 and also for WG2.

In addition to this main topic there was the report from the last WG9 and WG7 meetings as well as a short review of the next steps to be done toward our paper.

Meeting documentation

36th meeting, 13th of November 2023

Meeting date

13/11/2023 @ 5:00 PM (CET/CEST)

Meeting summary

After a short session of QUAREP-LiMi news and updates on our other running projects, we mainly focussed on our planned paper which should accompany the publishing of our protocols. We discussed main aspects, determined topics which need to be addressed and members who our responsible for working on the topics. To be able to have time to do so, we decide to skip the meeting in January 2024.

Meeting documentation

35th meeting, 10th of October 2023

Meeting date

10/10/2023 @ 5:00 AM (CET/CEST)

Meeting documentation

34th meeting, 11th of September 2023

Meeting date

11/09/2023 @ 5:00 PM (CET/CEST)

Meeting documentation

33rd meeting, 14th of August 2023

Meeting date

14/08/2023 @ 5:00 PM (CET/CEST)

Meeting documentation

32nd meeting, 10th of July 2023

Meeting date

10/07/2023 @ 5:00 PM (CET/CEST)

Meeting documentation

31st meeting, 12th of June 2023

Meeting date

12/06/2023 @ 5:00 PM (CET/CEST)

Meeting documentation

30th meeting, 8th of May 2023

Meeting date

08/05/2023 @ 5:00 PM (CET/CEST)

Meeting summary

1) First we discussed some general QUAREP-LiMi news:

– report from the WG9 & co-chairs meeting on May 2nd (feedback from general meeting, QUAREP-LiMi Organigram)

2) Second we talked about the preparation of our contribution for ELMI 2023:

– presenters, preparation support team members, dates for preparation meetings, abstract was sent, date for the actual workshop, which setups will be available

3)  Third, we review the annual meeting:

– intro session: needs to be improved, idea from WG9: have meeting schemes (Spring: external & outreach, Fall: internal – update about work group progress

– our break out room: comments to follow up (advice about when a detector is OK / not OK; make protocol available)

– main panel (great) & general feedback (general goal of QUAREP needs to be made more clear)

– our survey (short look at the results, need to be looked at closer later)

4)  Mathias reported from his EMVA1288 training (will optimize and upload the slides):

– report followed by discussion about how to use the material, how to adapt it to our LiMi needs, we always use EMVA1288 as guidance

5)  Last we postponed David’s report again, to the June meeting and agreed on the next steps.

Meeting documentation

Memberlist WG 2