WG 2 – Detection system performance

In short, the main topic of this meeting was to discuss a potential collaboration with the MetrologyJ_QC group. For that, Orestis and Julien joined us as guests.

We found that both our efforts are complementary and thus can nicely be combined. The MetrologyJ_QC analysis tool focusses on calculating camera read noise, DSNU (dark signal non-uniformity), and hot and cold pixels. Our WG2 analysis tool focusses on calculating the photon-conversion factor. In addition, we as WG2 aim to combine our analysis with an easy to use (automated) acquisition protocol.

Generally, QUAREP-LiMi needs to develop a nice overarching framework for running all our quality control scripts automatically together easily in one tool. Someone needs to take the lead for this endeavour. There is already great groundwork being done, thanks to Kees, for WG1 and also for WG2.

In addition to this main topic there was the report from the last WG9 and WG7 meetings as well as a short review of the next steps to be done toward our paper.

After a short session of QUAREP-LiMi news and updates on our other running projects, we mainly focussed on our planned paper which should accompany the publishing of our protocols. We discussed main aspects, determined topics which need to be addressed and members who our responsible for working on the topics. To be able to have time to do so, we decide to skip the meeting in January 2024.

1) First we discussed some general QUAREP-LiMi news:

– report from the WG9 & co-chairs meeting on May 2nd (feedback from general meeting, QUAREP-LiMi Organigram)

2) Second we talked about the preparation of our contribution for ELMI 2023:

– presenters, preparation support team members, dates for preparation meetings, abstract was sent, date for the actual workshop, which setups will be available

3)  Third, we review the annual meeting:

– intro session: needs to be improved, idea from WG9: have meeting schemes (Spring: external & outreach, Fall: internal – update about work group progress

– our break out room: comments to follow up (advice about when a detector is OK / not OK; make protocol available)

– main panel (great) & general feedback (general goal of QUAREP needs to be made more clear)

– our survey (short look at the results, need to be looked at closer later)

4)  Mathias reported from his EMVA1288 training (will optimize and upload the slides):

– report followed by discussion about how to use the material, how to adapt it to our LiMi needs, we always use EMVA1288 as guidance

5)  Last we postponed David’s report again, to the June meeting and agreed on the next steps.

Draft versions of the workgroup presentation and survey for the General assembly meeting were shared and suggestions for improvements were given. Discussion topics for the breakout session were given. Mathias’ report from his EMVA1288 training and the update on David MacFadden’s analysis script will be discussed during the meeting in May.

1. First we discussed some general QUAREP-LiMi news:
   • QUAREP-LiMi is now a community partner of image.sc, we potentially need to contribute to the moderating in the future
   • there is a community room at ELMI for QUAREP and other imaging communities to do 6 workshops, WG2 member contribution is welcome
   • the French MetrologyJ-QC group is very interested in a collaboration with us in WG2, we will invite them to a future WG2 meeting
   • WG12 paper is in the review process of Nature methods
2. Second we collected ideas on which topics to discuss in the breakout room. It was agreed to especially include open questions and problems: challenge how to measure sensitivity; our strategy of community roll-out; current problems we are facing: UUIDs; data handling, storage, and retrieval. We also collected some potential survey questions. A small team was formed to prepare the breakout room, the short introduction video and questions for a possible survey; all of which will be presented during our next WG2 meeting.
3. Third followed the reports from the small group measurement teams: for sample preparation, we now have the text for the marker-pen sample on protocols.io (please check it out). A text is planned for the Chromaslide ramp. MathiasH went to the EMVA1288 certification course. He will report in our next meeting.