WG 3 – Uniformity of field – flatness

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WG 3 – Objectives

Illumination field uniformity is critical for quantitative imaging when comparing fluorescence intensities across a field-of-view (FOV) or a large tile of images capturing an entire sample. If the illumination is not constant over a large area, the fluorescence intensities will not represent the inherent fluorescence but rather the location within the image. Thus, WG3 aims at defining a set of universal protocols to assess the uniformity of illumination (i.e., “flatness” of field) over the FOV of any photon-based imaging system and allow for correction of any non-uniformity. These protocols will identify the necessary tools, the procedures required to perform the measurements, and the analysis methods required for their interpretation. WG3 will also define criteria regarding the cut-off for acceptability and the need for correction. A database will be created with ideal images of uniform fields-of-view from different microscope modalities and settings to be used as a reference by the community and validate the protocol and criteria.

WG 3 – Co-Chairs

WG 3 - Latest News

We welcome a new member to our working groups 2, 3, 10, 11 and 12. Mr. Aleš Kladnik from, the Biotechnical Faculty of University of Ljubljana in Slovenia just joined QUAREP-LiMi.

We welcome our first member from the United Arab Emirates. Rachid Rezgui of NYU Abu Dhabi joined the Working Groups 1-7, 9, 10 and 13. Change the Quality of Light Microscopy Imaging as member of QUAREP-LiMi join us here.

We welcome our first member from Qatar. Doha Abbirami of Sidra Medicine joined the Working Groups 3, 8, 9, 10, 11 and 12. Change the Quality of Light Microscopy Imaging as member of QUAREP-LiMi join us here.

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13/03/2025 @ 5:00 PM (CET/CEST)

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13/02/2025 @ 5:00 PM (CET/CEST)

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12/09/2024 @ 5:00 PM (CET/CEST)

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09/04/2024 @ 5:00 PM (CET/CEST)

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12/03/2024 @ 5:00 PM (CET/CEST)

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