WG 3 – Objectives
Illumination field uniformity is critical for quantitative imaging when comparing fluorescence intensities across a field-of-view (FOV) or a large tile of images capturing an entire sample. If the illumination is not constant over a large area, the fluorescence intensities will not represent the inherent fluorescence but rather the location within the image. Thus, WG3 aims at defining a set of universal protocols to assess the uniformity of illumination (i.e., “flatness” of field) over the FOV of any photon-based imaging system and allow for correction of any non-uniformity. These protocols will identify the necessary tools, the procedures required to perform the measurements, and the analysis methods required for their interpretation. WG3 will also define criteria regarding the cut-off for acceptability and the need for correction. A database will be created with ideal images of uniform fields-of-view from different microscope modalities and settings to be used as a reference by the community and validate the protocol and criteria.
WG 3 – Co-Chairs
Call:
+49 89 8578 3971
Email:
spitaler@biochem.mpg.de
Martin Spitaler
WG 3 - Latest News
Last meeting: 12th March 2026, 5:00 pm
Memberlist WG 3
Last name First Name Town Organisation Affiliation Lab Affiliation Country Time Zone
af Klinteberg
Claes
Lund
Lumito AB
Industry
SE
UTC+1
Alshafie
Walaa
Ashburn
Janelia research campus
Univ.
US
UTC-4
Avilov
Sergiy
Freiburg
Max Planck Institute of Immunobiology and Epigenetics
MPG
DE
UTC+2
Azevedo
Maria
Porto
i3S/University of Porto
Univ.
PT
UTC+1
Bagley
Steve
Manchester
University of Manch.
Univ.
GB
UTC+1
Banerjee
Priyam
New York
Rockefeller University
Univ.
US
UTC-5
Bosch
Manel
Barcelona
University of Barcelona CCITUB
Univ.
ES
UTC+2
Bourg
Nicolas
Cachan
Abbelight
Industry
FR
UTC+1
Braunstein
Thomas
København N
Kbh. Uni. CFIM
Univ.
DK
UTC-1
Broeking
Kai
Goettingen
MPI
MPG
DE
UTC+2
Brunt
Peter
Fairport
AVR Optics
Industry
US
UTC-5
Burke
Russell
Beaverton
Lumencor
Industry
US
UTC-8
Campbell
George
Memphis
St. Jude Children’s Research Hospital
Private lab
US
UTC-6
Carvalho
Mariana
Braga
INL – International Iberian Nanotechnology Laboratory
EU-Org
PT
UTC+-0
Catrina
Irina
New York
Yeshiva University
Univ.
US
UTC-5
Ceccarelli
Raino
Rome
CrestOptics S.p.A.
Industry
IT
UTC+1
Chacko
Jenu
Madison
University of Wisconsin-Madison
Univ.
US
UTC-6
Cialowicz
Katarzyna
New York
Icahn School of Medicine at Mount Sinai
Univ.
US
UTC-5
Clément
Jean-Emmanuel
Villeneuve d’Ascq cedex
University of lille
Univ.
FR
UTC+2
Cole
Louise
Sydney
University of Technology
Univ.
AU
UTC+11
Cole
Richard
Albany
New York State Dept of Health, Wadsworth Center
Gov.
US
UTC-7
Dauphin
Aurelien
Paris
RTmfm
Univ.
FR
UTC+2
Dencevski
Aleksa
Belgrade
Institute of physics Belgrade
Univ.
CS
UTC+1
Diez-Guerra
F. Javier
Madrid
CBMSO (CSIC-UAM)
Univ.
ES
UTC+2
Duwé
Sam
Diepenbeek
Universiteit Hasselt
Univ.
BE
UTC+1
Ecker
Rupert
Vienna
TissueGnostics GmbH
Univ.
AT
UTC+1
Eseola
Alice
Norwich
The Sainsbury Laboratory
Univ.
GB
UTC+1
Esner
Milan
Brno
Ceitec Masaryk University
Univ.
CZ
UTC+1
Fasoli
Anna
Davis
89 North
Industry
US
UTC-8
Ferdous
Zannatul
Sapporo
Hokkaido University
Univ.
JP
UTC+9
Fujimori
Toshihiko
Okazaki
JP BioImaging
Gov.
JP
UTC+9
Geißler
Daniel
Berlin
PolyAn GmbH
Industry
DE
UTC+1
Girard
Philippe
Paris
Institut Jacques Monod – Université de Paris
Univ.
FR
UTC+1
Goda
Kazuhito
Hachioji
EVIDENT
Industry
JP
UTC+9
Gramann
Alex
Andover
CoolLED Ltd.
Industry
GB
UTC+-0
Greenwood
Harrison
London
The Francis Crick Institute
Univ.
GB
UTC+-0
Grunwald
David
Worcester
University of Massachusetts
Univ.
US
UTC-4
Gudavicius
Dominykas
Vilnius
Light Conversion
Industry
LT
UTC+2
Hannan
Riley
Charlottesville
University of Virginia
Univ.
US
UTC-5
Hassani
Ibrahim
Amsterdam
Nikon BV
Industry
NL
UTC+2
Hatto
Mao
Prague
Nikon
Industry
CZ
UTC+2
Herzog
Dylan
London
Microscopy Innovation Centre, King’s College London
Univ.
GB
UTC+-0
Inavalli
V V G
Southampton
University of Southampton
Univ.
GB
UTC+-0
Joyal
Mathieu
Ottawa
University of Ottawa
Univ.
CA
UTC-4
Kaiping
Arnica
Freiburg
University of Freiburg
Univ.
DE
UTC+2
Key
Jillian
Ottawa
University of Ottawa
Univ.
CA
UTC-4
Kirchenbüchler
David
Chicago
Northwestern
Univ.
US
UTC-6
Kladnik
Aleš
Ljubljana
University of Ljubljana, Biotechnical Faculty
Univ.
SI
UTC+1
Köning
Rainer
Braunschweig
PTB Braunschweig
Gov.
DE
UTC+1
Krzic
Uros
Munich
Carl Zeiss Microscopy GmbH
Industry
DE
UTC+2
Kumar
Purnima
CAMBRIDGE
Gurdon Institute, University of Cambridge
Univ.
GB
UTC+-0
Lahlou
Aliénor
PARIS
Sony CSL – Paris
Univ.
FR
UTC+1
Lai
Pok Yui
Oxford
University of Oxford
Univ.
GB
UTC+1
Lai
Queenie
London
The Institute of Cancer Research
Univ.
GB
UTC+-0
Laktiushkin
Viktor
Sochi
Sirius
Univ.
RU
UTC+3
Landua
Kalika
Houston
Nikon Instruments
Industry
US
UTC-5
Lee
Kitty
Stanford
Stanford University
Univ.
US
UTC-8
Lee
Woei Ming Steve
Canberra
John Curtin School of Medical Research, The Australian National University
Univ.
AU
UTC+10
Levitt
James
London
Nikon Imaging Centre, King’s College London, UK
Univ.
GB
UTC+-0
Li
Xiaoming
Shanghaitech
Shanghaitech University
Univ.
CN
UTC+8
Liu
Tristan
New York
Columbia University
Univ.
US
UTC-4
Lorentz
Pascal
Basel
Department of Biomedicine University of Basel
Univ.
CH
UTC+2
Louis
Boris
Leuven
KU Leuven
Univ.
BE
UTC+1
Manning
Lane
Fairport
AVR Optics
Industry
US
UTC-5
Mast
Fred
Seattle
Seattle Children’s Hospital
Univ.
US
UTC+8
Micu
Ileana
Belfast
Queen’s University Belfast
Univ.
GB
UTC+1
Monterroso
Baptiste
Nice
University of Nice
Univ.
FR
UTC+2
Mulholland
Jonathan
Stanford
Stanford University
Univ.
US
UTC-7
Munck
Sebastian
Leuven
University of Leuven
Univ.
BE
UTC+2
Narvaez
William
Lastenia Toral
NGSI
Private lab
EC
UTC-5
Nechyporuk-Zloy
Volodymyr
London
Imperial College of Science, Technology and Medicine
Univ.
GB
UTC+-0
Nitschke
Roland
Freiburg
University of Freiburg, Life Imaging Center
Univ.
DE
UTC+2
Oord
Kees
Amsterdam
Nikon
Industry
NL
UTC+1
Oreopoulos
John
Toronto
Oxford Inst. Andor
Industry
CA
UTC-5
Orlova
Natalia
Seattle
Allen Institute
Private lab
US
UTC-7
Oster
Markus
Munich
MPI of Biochemistry
MPG
DE
UTC+2
Parel
Emma
Cape Town
University of Cape Town
Univ.
ZA
UTC+2
Pattison
Allister
Belfast
Andor
Industry
GB
UTC+-0
Peng
Tingying
Neuherberg
Helmholtz Munich
Helmholtz
DE
UTC+1
Perin
Paola
Pavia
University of Pavia
Univ.
IT
UTC+1
Peterka
Darcy
New York
Columbia University
Univ.
US
UTC+5
Petrov
Maxim
Yekaterinburg
SIAMS Ltd
Industry
RU
UTC+5
Reither
Sabine
Basel
Friedrich Miescher Institute for Biomedical Research
Private lab
CH
UTC+2
Rexhepaj
Elton
Paris
Sanofi
Industry
FR
UTC+1
Ritz
Sandra
Mainz
Institute of Molecular Biology – IMB
Univ.
DE
UTC+2
Saint-Hilaire
Cadisha
Paris
Curie Institute
Univ.
FR
UTC+1
Sanetrnik
Vojtech
Brno
Telight
Industry
CZ
UTC+1
Sathappan
Abbirami
Doha
Sidra Medicine
Gov.
QA
UTC+3
Sauer
Ryan
Williston
89 North, Inc.
Industry
US
UTC-4
Schapman
Damien
Rouen
Normandy University
Univ.
FR
UTC+2
Schmied
Jürgen
Gräfelfing
GATTAquant GmbH
Industry
DE
UTC+2
Schreiner
Ryan
New York
Weill Cornell Medicine
Univ.
US
UTC-4
Schuchardt
Chris
Jena
Carl Zeiss Jena GmbH
Industry
DE
UTC+1
Segal
Gabriela
Melbourne
University of Melbourne
Univ.
AU
UTC+10
Selbach
Florian
Gräfelfing
GATTAquant GmbH
Industry
DE
UTC+2
Shakouri
Mahsa
Heidelberg
DKFZ
Industry
DE
UTC+12
Shemesh
Michal
Delft
TUD
Univ.
NL
UTC+1
Silván Alcaraz
Ágata
Copenhagen
University of Copenhagen
Univ.
DK
UTC+1
Simm
Alan
Liverpool
Liverpool John Moores University
Univ.
GB
UTC+-0
Spitaler
Martin
Munich
MPI of Biochemistry
MPG
DE
UTC+2
Srinivasan
Sathya
Beaverton
ONPRC
Univ.
US
UTC-8
Stifani
Nicolas
Montréal
Université de Montréal
Univ.
CA
UTC-5
Tan
Lihao
Singapore
Venture Biotech Modules Business Private Limited
Industry
SG
UTC+8
Tapia
Pablo
Bethesda
National Institutes of Health (NIH)
Gov.
US
UTC-4
Thomson
Darren
Exeter
University of Exeter
Univ.
GB
UTC+1
Thuenauer
Roland
Hamburg
Heinrich Pette Institute
Leibniz
DE
UTC+2
Titus
Steve
Rising SUn
ThermoFisher
Industry
US
UTC-5
Tu
Haohua
Urbana
University of Illinois at Urbana-Champaign
Univ.
US
UTC-6
Valiya Peedikakkal
Liyana
Oxford
Oxford Nanoimaging
Industry
GB
UTC+1
van der Voort
Hans
Hilversum
Scientific Volume Imaging BV
Industry
NL
UTC+1
van Oostende-Triplet
Chloë
Ottawa
University of Ottawa
Univ.
CA
UTC-5
Wee
Erika
Cold Spring Harbor
Cold Spring Harbor Laboratory
Univ.
US
UTC-5
Węgierski
Tomasz
Warsaw
International Institute of Molecular and Cell Biology
Gov.
PL
UTC+2
Wilde
Geraint
Belfast
Oxford Inst. Andor
Industry
GB
UTC+1
Zhou
Chunyuan
Pudong New Area
Nikon Instruments (Shanghai) Co.,Ltd
Industry
CN
UTC+8