WG 1 – Objectives
Comparison of fluorescence intensities between images requires measurements of the illumination power and stability of the excitation light source. WG1 aims at establishing a recommended protocol for measuring the stability of a light source during both short- and long-term image acquisition sessions using calibrated external power sensors. This initial aim will be extended later to measure the absolute flux of light through the illumination path and irradiance of the sample. The initial protocol will be designed around lasers on confocal microscopy platforms (raster scanning and spinning disks). It will be later modified towards other microscopy techniques (widefield, TIRF, light-sheet, super-resolution).
WG 1 – Co-Chairs
Call:
+1 (913) 562 7261
Luis Acevedo
Co-Chair of WG 1 and WG 15
Call:
+1 501 409 1486
Email:
nathalieg@alleninstitute.org
Nathalie Gaudreault
Co-Chair of WG 1
WG 1 - Latest News
Last meeting: 7th May 2025, 2:15 pm
Release of protocol “Illumination power and illumination stability measurements for contocal, widefield and multiphoton microscopes“.
Beside step-by-step instructions, the protocol provides:
- A link to a public database with measurements made by members of our group and to which you can contribute by uploading your own measurements.
- A link to a GitHub repository with scripts to automatize the measurements with some scopes and power meters
- A link to tutorial videos which are available on the QUAREP-LiMi YouTube Channel
- Automation
- Aims: The QUAREP-LiMi power and stability assessment protocol requires long and repetitive measurements for each light source as other parameters are tested. The aim of this subgroup is to provide feasible means for carrying out these processes, taking advantage of the automation capabilities of modern microscopes.
- Co-leads: Mišo Mitkovski, Nasser Darwish
- On-going tasks:
- Published scripts and instructions for Nikon and Zeiss microscopes in the QUAREP-LiMi Github repository.
- Cloud storage location (can be accessed as a member) → here
- Environment
- Glossary
- Power Meter
Memberlist WG 1
Last name First Name Town Organisation Affiliation Lab Affiliation Country Time Zone
Acevedo
Luis
Overland Park
thya
Industry
US
UTC-6
Alshafie
Walaa
Ashburn
Janelia research campus
Univ.
US
UTC-4
Avilov
Sergiy
Freiburg
Max Planck Institute of Immunobiology and Epigenetics
MPG
DE
UTC+2
Bagley
Steve
Manchester
University of Manch.
Univ.
GB
UTC+1
Bammann
Rodrigo
Uckfield
Scientifica
Industry
GB
UTC+1
Banerjee
Priyam
New York
Rockefeller University
Univ.
US
UTC-5
Barachati
Fábio
Berlin
PicoQuant GmbH
Industry
DE
UTC+1
Barna
Laszlo
Bloomington
Indiana University Bloomington
Gov.
HU
UTC+1
Bennett
Sarah
Coventry
University of Warwick
Univ.
GB
UTC+1
Bergles
Eric
San Jose
Pavilion Integration Corporation
Industry
US
UTC-8
Birngruber
Konstantin
Munich
Toptica
Industry
DE
UTC+2
Boczonadi
Veronika
Newcastle
Newcastle University
Univ.
GB
UTC+-0
Boehm
Ulrike
Oberkochen
Carl Zeiss AG
Industry
DE
UTC-4
Bosch
Manel
Barcelona
University of Barcelona CCITUB
Univ.
ES
UTC+2
Bottomley
Amy
Ultimo
University of Technology Sydney
Univ.
AU
UTC+10
Boxall
Sally
Leeds
University of Leeds
Univ.
GB
UTC+1
Brideau
Craig
Calgary
University of Calgary
Univ.
CA
UTC-7
Brunt
Peter
Fairport
AVR Optics
Industry
US
UTC-5
Calvo
Maria
Barcelona
University of Barcelona
Univ.
ES
UTC+-0
Carvalho
Mariana
Braga
INL – International Iberian Nanotechnology Laboratory
EU-Org
PT
UTC+-0
Casse
Alhassan
Vitry-sur-Seine
SANOFI
Industry
FR
UTC+2
Ceccarelli
Raino
Rome
CrestOptics S.p.A.
Industry
IT
UTC+1
Chacko
Jenu
Madison
University of Wisconsin-Madison
Univ.
US
UTC-6
Chatterjee
Nabanita
Gothenburg
University of Gothenburg
Univ.
SE
UTC+1
Chinnaraj
Subash Bommu
Amstelveen
Nikon Europe B.V.
Industry
NL
UTC+1
Cialowicz
Katarzyna
New York
Icahn School of Medicine at Mount Sinai
Univ.
US
UTC-5
Colarusso
Pina
Calgary
University of Calgary
Univ.
CA
UTC-7
Cole
Louise
Sydney
University of Technology
Univ.
AU
UTC+11
Cole
Richard
Albany
New York State Dept of Health, Wadsworth Center
Gov.
US
UTC-7
Darwish
Nasser
Klosterneuburg
Institute of Science and Technology Austria
Gov.
AT
UTC+1
Dervish
Suat
Westmead
Westmead Institute for Medical Research
Gov.
AU
UTC+10
Dietzel
Ralf
Rodgau
Omicron
Industry
DE
UTC+2
Diez-Guerra
F. Javier
Madrid
CBMSO (CSIC-UAM)
Univ.
ES
UTC+2
Drent
Peter
Amsterdam
ONI
Industry
NL
UTC+1
Duwé
Sam
Diepenbeek
Universiteit Hasselt
Univ.
BE
UTC+1
Egron
Audrey
Pessac
Argolight
Industry
FR
UTC+1
Eismann
Frank
Jena
Carl Zeiss Microscopy GmbH
Industry
DE
UTC+2
Eseola
Alice
Norwich
The Sainsbury Laboratory
Univ.
GB
UTC+1
Esner
Milan
Brno
Ceitec Masaryk University
Univ.
CZ
UTC+1
Faklaris
Orestis
Montpellier
RTmfm
Univ.
FR
UTC+2
Fallisch
Arne
Freiburg
University of Freiburg, Life Imaging Center
Univ.
DE
UTC+1
Felscher
Andreas
Luebeck
Coherent
Industry
DE
UTC+2
Ferdous
Zannatul
Sapporo
Hokkaido University
Univ.
JP
UTC+9
Filesi
Federico
Rome
CrestOptics S.p.A.
Industry
IT
UTC+1
Forbes
Ashli
Vancouver
University of British Columbia
Univ.
CA
UTC-8
Fu
Yangting
Bonn
Deutsches Zentrum für Neurodegenerative Erkrankungen e. V. (DZNE)
Helmholtz
DE
UTC+1
Fuhrhop
Carlos
Valdivia
Universidad Austral de Chile
Univ.
CL
UTC+4
Gaudreault
Nathalie
Seattle
Allen Institute
Private lab
US
UTC-7
Gelman
Laurent
Basel
Friedrich Miescher Institute for Biomedical Research
Private lab
CH
UTC+2
Giakoumakis
Nikolaos
Barcelona
Institute for Research in Biomedicine Barcelona
Univ.
ES
UTC+1
Gonnert
Manfred
Bergkirchen
Thorlabs GmbH
Industry
DE
UTC+2
Goodhand
Isabel
Andover
CoolLED Ltd.
Industry
GB
UTC+-0
Gramann
Alex
Andover
CoolLED Ltd.
Industry
GB
UTC+-0
Greenwood
Harrison
London
The Francis Crick Institute
Univ.
GB
UTC+-0
Grunwald
David
Worcester
University of Massachusetts
Univ.
US
UTC-4
Gu
Tingting
Norman
University of Oklahoma
Univ.
US
UTC-6
Guaimas
Francisco
San Martin
CONICET-UNSAM
Gov.
AR
UTC-3
Gudavicius
Dominykas
Vilnius
Light Conversion
Industry
LT
UTC+2
Gunkel
Manuel
Heidelberg
EMBL Heidelberg
EU-Org
DE
UTC+2
Hannan
Riley
Charlottesville
University of Virginia
Univ.
US
UTC-5
Hartley
Matthew
Cambridge
EMBL-EBI
EU-Org
GB
UTC+1
Hatto
Mao
Prague
Nikon
Industry
CZ
UTC+2
Herzog
Dylan
London
Microscopy Innovation Centre, King’s College London
Univ.
GB
UTC+-0
Hibbs
Ben
Silvan
Klein Australia Pty Ltd
Industry
AU
UTC+10
Hoffmann
Birgit
Jena
UKJ
Univ.
DE
UTC+2
Ibarra
Humberto
New York
Zuckerman Institute – University of Columbia
Univ.
US
UTC-4
Iremonger
James
Edinburgh
University of Edinburgh
Univ.
GB
UTC+-0
Jaffe
Claudia
Beaverton
Lumencor, Inc.
Industry
US
UTC-7
Joyal
Mathieu
Ottawa
University of Ottawa
Univ.
CA
UTC-4
Kasper
Robert
Hamburg
Evident Europe GmbH
Industry
DE
UTC-1
Key
Jillian
Ottawa
University of Ottawa
Univ.
CA
UTC-4
Kirchenbüchler
David
Chicago
Northwestern
Univ.
US
UTC-6
Kirchner
Marcel
Cologne
MPI
MPG
DE
UTC+2
Köbele
Luis
Freiburg
opto biolabs GmbH
Industry
DE
UTC+1
KOKSOY
AYSE
Houston
MD Anderson CC
Univ.
US
UTC-6
Köning
Rainer
Braunschweig
PTB Braunschweig
Gov.
DE
UTC+1
Krens
Gabriel
Klosterneuburg
Institute of Science and Technology Austria
Gov.
AT
UTC+1
Kumar
Purnima
CAMBRIDGE
Gurdon Institute, University of Cambridge
Univ.
GB
UTC+-0
Lahlou
Aliénor
PARIS
Sony CSL – Paris
Univ.
FR
UTC+1
Lai
Pok Yui
Oxford
University of Oxford
Univ.
GB
UTC+1
Laktiushkin
Viktor
Sochi
Sirius
Univ.
RU
UTC+3
Landua
Kalika
Houston
Nikon Instruments
Industry
US
UTC-5
Laude
Alex
Newcastle
Newcastle University
Univ.
GB
UTC+1
Leavesley
Silas
Mobile
University of South Alabama
Univ.
US
UTC-5
Leburn
Christopher
Edinburgh
Chromacity
Univ.
GB
UTC-1
LeDue
Jeffrey
Vancouver
University of British Columbia
Univ.
CA
UTC-7
Lee
James
Houston
Baylor College of Medicine
Univ.
US
UTC-6
Lee
Woei Ming Steve
Canberra
John Curtin School of Medical Research, The Australian National University
Univ.
AU
UTC+10
Lees
Robert
Didcot
Science and Technology Facilities Council
Gov.
GB
UTC+-0
Lerksuthirat
Tassanee
Rachathewi
Mahidol Unive
Univ.
TH
UTC+7
Levitt
James
London
Nikon Imaging Centre, King’s College London, UK
Univ.
GB
UTC+-0
Li
Xiaoming
Shanghaitech
Shanghaitech University
Univ.
CN
UTC+8
Lin
Sean
Williston
89 North, Inc.
Industry
US
UTC-4
Liu
Tristan
New York
Columbia University
Univ.
US
UTC-4
Lopes
Alexandre
Oeiras
IGC
Private lab
PT
UTC+-0
Lorentz
Pascal
Basel
Department of Biomedicine University of Basel
Univ.
CH
UTC+2
Luccardini
Camilla
Lyon
CIQLE Centre d’Imagerie Quantitative Lyon Est
Univ.
FR
UTC+1
Machovina
Tyler
Sewickley
Okolab
Industry
US
UTC+5
Madi
Mehdi
Cachan
Abbelight
Industry
FR
UTC+1
Manning
Lane
Fairport
AVR Optics
Industry
US
UTC-5
Mathur
Aastha
Heidelberg
Euro-BioImaging
EU-Org
DE
UTC+2
Micu
Ileana
Belfast
Queen’s University Belfast
Univ.
GB
UTC+1
Mitkovski
Miso
Göttingen
GerBi-GMB WG 1, MPI-NAT
MPG
DE
UTC+2
Monterroso
Baptiste
Nice
University of Nice
Univ.
FR
UTC+2
Moore
Josh
Dundee
University of Dundee
Univ.
GB
UTC+1
Munck
Sebastian
Leuven
University of Leuven
Univ.
BE
UTC+2
Narvaez
William
Lastenia Toral
NGSI
Private lab
EC
UTC-5
Nechyporuk-Zloy
Volodymyr
London
Imperial College of Science, Technology and Medicine
Univ.
GB
UTC+-0
Nelson
Michael
Madison
University of Wisconsin
Univ.
US
UTC-8
Nitschke
Roland
Freiburg
University of Freiburg, Life Imaging Center
Univ.
DE
UTC+2
Oord
Kees
Amsterdam
Nikon
Industry
NL
UTC+1
Orlova
Natalia
Seattle
Allen Institute
Private lab
US
UTC-7
Ossato
Giulia
Mannheim
Leica
Industry
DE
UTC+1
Peterka
Darcy
New York
Columbia University
Univ.
US
UTC+5
Petrov
Eugene
Berlin
Bundesanstalt für Materialforschung und -prüfung
Gov.
DE
UTC+1
Repula
Andrii
Beaverton
Lumencor, Inc.
Industry
US
UTC+7
Resch-Genger
Ute
Berlin
BAM
Gov.
DE
UTC+2
Ronkainen
Veli-Pekka
Oulu
University of Oulu
Univ.
FI
UTC+2
Saint-Hilaire
Cadisha
Paris
Curie Institute
Univ.
FR
UTC+1
Sauer
Ryan
Williston
89 North, Inc.
Industry
US
UTC-4
Schmidt
Alfonso
Kelburn Parade
Malaghan Institute of Medical Reserach
Private lab
NZ
UTC+12
Schreiner
Ryan
New York
Weill Cornell Medicine
Univ.
US
UTC-4
Schroth-Diez
Britta
Dresden
MPI-CBG
MPG
DE
UTC+2
Schwartz
Stanley
Austin
Nikon
Industry
US
UTC-5
Segal
Gabriela
Melbourne
University of Melbourne
Univ.
AU
UTC+10
Shakouri
Mahsa
Heidelberg
DKFZ
Industry
DE
UTC+12
Shemesh
Michal
Delft
TUD
Univ.
NL
UTC+1
Siew
Ronian
Singapore
Venture Biotech Modules Business Private Limited
Industry
SG
UTC-8
Simm
Alan
Liverpool
Liverpool John Moores University
Univ.
GB
UTC+-0
Srinivasan
Sathya
Beaverton
ONPRC
Univ.
US
UTC-8
Stats
Sam
Williston
89 North, Inc.
Industry
US
UTC-5
Stifani
Nicolas
Montréal
Université de Montréal
Univ.
CA
UTC-5
Tan
Lihao
Singapore
Venture Biotech Modules Business Private Limited
Industry
SG
UTC+8
Tapia
Pablo
Bethesda
National Institutes of Health (NIH)
Gov.
US
UTC-4
Tassis
Kostas
Leiden
Leiden University
Univ.
NL
UTC+1
Thuenauer
Roland
Hamburg
Heinrich Pette Institute
Leibniz
DE
UTC+2
Türschmann
Pierre
Erlangen
Interherence GmbH
Industry
DE
UTC+1
Tzavaras
Nikos
New York
Icahn School of Medicine at Mount Sinai
Univ.
US
UTC-5
Valiya Peedikakkal
Liyana
Oxford
Oxford Nanoimaging
Industry
GB
UTC+1
van Oostende-Triplet
Chloë
Ottawa
University of Ottawa
Univ.
CA
UTC-5
Wee
Erika
Cold Spring Harbor
Cold Spring Harbor Laboratory
Univ.
US
UTC-5
Weiss
Kurt
Madison
University of Wisconsin-Madison
Univ.
US
UTC-6
Wilde
Geraint
Belfast
Oxford Inst. Andor
Industry
GB
UTC+1
Zeiss
Erich
Beaverton
Lumencor, Inc.
Industry
US
UTC-5
Zuschratter
Werner
Magdeburg
Leibniz-Institut für Neurobiologie – LIN
Leibniz
DE
UTC+2