WG 1 – Illumination power

We had a total of 10 participants. We have finalized Version 3 of our protocol on 2P extension. We have resolved all comments and added a new disclaimer. Our next step is to align with relevant WG and ask them to review and endorse our protocol. Nathalie will prepare a short presentation and will send instructions to the relevant co-chairs. We should complete this part of the review process by the end of October. We have a draft template to structure the manuscript on Irradiance measurements. Please review it before our next meeting. Our next meeting will be on, Wednesday, November 6th, same time, same Zoom link.

We had a total of 14 participants. Luis is now co-chairing WG1 with Nathalie. We are finalizing Version 3 of our protocol on 2P extension. Laurent has been spearheading this effort. In the second part of the meeting, we discussed and proposed to write a manuscript to educate biologists on irradiance measurements. We will start drafting a skeleton and template for the manuscript at our next meeting. Our next meeting will be on, Wednesday, October 2nd, same time, same Zoom link. The meeting calendar invite series (every first Wednesday of the month) has been sent to everyone.   You can find the complete minutes and the recording of the meeting here. Quick announcement: Laurent shared with us a new peer-reviewed journal that could be a good place for many QUAREP protocols and communications. Methods of Microscopy magazine registration, it is free. The link to the journal can be found here. The SC committee invites you to review the Update of Best Practices document (https://nextcloud.quarep.org/index.php/f/469900) and provide feedback and suggestions. There will be a panel discussion on image data sharing at ASCB 2024 in San Diego. We believe this session will be of great interest and relevant to all industry partners. If you are attending please let Nathalie know and contact her if you want more information.

A small group (9 of us) met to discuss some logistics. We will not meet in August and observe the summer break vacations. We will reconvene in September to complete V3 of our illumination stability protocol and will begin meeting to develop a protocol and guidelines for the measurement of irradiance. Luis Acevedo has volunteered to be a co-chair of WG1 with Nathalie. TingTing has volunteer to represent WG1 at the OB. We still need someone to volunteer to represent WG1 at the ERB. Nathalie will represent WG1 at the SC. The minutes and recoding of the meeting can be found here.

We had a short round of presentation for new comers. We decided to pause just this time our work on our protocol and 2-photon microscopy modality to focus on irradiance, and had the pleasure to listen to two very nice presentations by Ronian Siew and then Alienor Lahlou. For those of you who missed it, the recordings can be found here.

We summarized the By-Laws’ presentation since everyone had seen it at least once. The by-laws and its accompanying documents (Code of Conduct – CoC, Terms of Reference – ToR, and Best Practices – BP) aim to ensure QUAREP-LiMi provides a diverse, equitable, inclusive and transparent environment for its community to gather, address and efficiently make decision to critical challenges. You can find previous recordings of the By-Laws presentation from WG2 here, as well as the slides here. In the folder named Decision Making Discussion https://nextcloud.quarep.org/index.php/f/445127 you will find the other relevant documents related to the by-laws: •BP_BestPractices for QUAREP-LiMi Operations.docx •CoC_WorkGroups.docx •Bylaws Draft_04_a.docx Here are our action items: 1.      WG1 needs to appoint one representative for the SC, ERB, and OB (two-year terms). 2.      WG1 needs to elect its co-chairs for the next two-year term. Nathalie and Laurent are happy to continue on for another two year. We would love to have a third co-chair. 3.      We need candidates! – Let us know if you are interested in being our WG1 representative for SC (ideally one of the co-chairs), ERB, or OB, or if you want to become a WG1 co-chair before March 12^th. We also briefly brainstormed on known ways to measure irradiance and decided that a path forward would be to review a proposed definition (by WG13) and present a manuscript (journal club style) at our next meeting. WG1 will meet again on the 3^rd of April and will then continue expanding the Illumination power stability protocol to multi-photons.

First, our collaboration with WG-13 has been defined. Philippe Laissue (co-chair of WG13) talked about the mission of WG13 and how a protocol on how to measure irradiance could fit within the guidance on phototoxicity. Our first goal will be to define irradiance and write a small paragraph to summarize existing methods and guidelines on irradiance. Please share all references you have in this folder or directly in Zotero. We will define irradiance and discuss papers found at our next WG1 meeting (30 min each month). We then briefly discussed Robert Lees very relevant preprint on bioRxiv. We also made headway with version 3 of our protocol and included multi-photons specific comments and recommendations to steps 4 to 6 when needed. We will continue with step 7 at our next meeting. Please go to the protocol online here and add your comments and suggestions to the first 6 steps of the protocol. We will discuss all new comments at our next meeting.

During this meeting, we branched version 2 of our protocole to extrend it to Multi-photon systems. We discussed and modified staps 1 to 3 of the protocol.

Lin Yangchen presented his protocol and tools for “Irradiance Measurement on a spinning-disk confocal and TIRF microscope”. We decided to extend next time our protocol to 2P microscopy modality, and we collected the names of people in WG1 who want to collaborate with WG13 to create protocols on irradiance measurement and assessment of phytotoxicity. If you want also to join this subgroup, please contact Natalie Gaudreault.

Andrii Repula, Product Support Engineer at Lumencor, presented his white paper and how they measure light source stability at his company.

The subgroup for automation has developed a new script for Zeiss instrument that makes the old one obsolete. It will be uploaded on the website soon. Other participants also inform that more scripts for Leica and Visitron are being developed. We also all agreed on the fact that the GitHub is a living repository and will evolve with time, even when the protocols are done. We then reviewed the latest comments on the protocol and made small improvements to it. We decide to keep the stability formula as it is, even though it might be very sensitive to one outlier and does not reflect always what is wrong. However, we agree that to know there is an outlier or something is wrong is already very important, and one has to plot the stability curve anyhow, and can thereby immediate see why the stability value might be low. Nathalie explains the process for co-authorship and endorsement. We will ask WG chairs to read and say is they want to co-sign or endorse “works for me” through protocol.io. QUAREP general assembly: Laurent will prepare a presentation. Survey: we will align with Roland.