Britta Schroth-Diez, Kees van der Oorda Nikon Europe B.V.
b Light Microscopy Facility, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden Germany
The QUAREP-LiMi Tool Kit [1] was developed by the QUAREP-LiMi community to reduce the time needed to run periodic quality assessment protocols and organize the performance data acquired. During this workshop we will present its basic features and latest developments.
In accordance to the protocol defined by the QUAREP-LiMi WG 1 [2] the Tool Kit integrates user friendly interfaces for the assessment of illumination power and stability. These interfaces are already available for the Nikon and Zeiss acquistion software.
In addition, the Tool Kit includes support for the protocols for detector gain, dynamic range and noise assessment developed by the QUAREP-LiMi WG2 [2].
A highlight of the Tool Kit is its data browser. Thanks to the organized presentation of the performance metrics it is easier to diagnose problems – e.g. a small but steady power decrease over months– and take corrective and preventive actions before they become detrimental.
The Tool Kit functionality will be demonstrated during the workshop, where we will emphasize important details of the protocols.
[1] https://github.com/QUAREP-LiMi/QUAREP-LiMi-Tool-Kit
[2] https://www.protocols.io/workspaces/quarep-limi/publications