LiMi-Model for Imaging Scientists

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The LiMi Model helps microscopy users & scientists standardize data quality and reproducibility

Microscopy users and imaging scientists benefit from the LiMi Model’s clear, community-driven framework for recording the metadata that matters most—from sample preparation and imaging conditions to microscope hardware and acquisition settings. By integrating the LiMi-Model into your workflows, you ensure that your image data is not only reproducible and high quality but also compliant with FAIR principles, making it easier to share, publish, and reanalyse.

The LiMi-Model community is actively working to simplify its integration with popular microscopy tools like OMERO, Micro-Meta App, and OME-NGFF, helping you document experiments efficiently and completely without disrupting acquisition routines. Embracing the LiMi-Model enhances the scientific value of your datasets and aligns your work with emerging expectations from journals, funders, and core facilities.

Resources:
Metrology images – QUAREP maintains an OMERO database for storing images relevant to its work on quality control and reproducibility

Protocols – QUAREP has released several specific QC protocols on protocols.io

Publication guidelinesNature Cell Biology and other NPG journals now enforce a minimum reporting checklist based on the LiMi-Model

ISO standards for light microscopy metadata are under development

Bibliography:
The LiMi-Model- https://doi.org/10.1038/s41592-021-01327-9
OME-NGFF- https://www.nature.com/articles/s41592-021-01326-w
OMERO- https://www.nature.com/articles/nmeth.1896
Micro-Meta App- https://www.nature.com/articles/s41592-021-01315-z 

"Macroscopic data", NFDI4BIOIMAGE Consortium (2024): NFDI4BIOIMAGE data management illustrations by Henning Falk, Zenodo, https://doi.org/10.5281/zenodo.14186100, is used under a CC-BY 4.0 license. Modifications to this illustration include cropping