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10th meeting, 19th of October 2021
Meeting summary
We are currently generating data in several locations and with several different confocal microscopes of the WG4 members. We defined three workflows to analyze the data with the aim to specify contributions of the analysis workflow on the results. We decided to use the suggestions (Meta App) from WG7 (metadata) to save all our metadata. Matadata will be extremely important to discuss our results, thus we established a set of metadata important for WG4 and collect them in a table for future analysis.
8th meeting, 20th of July 2021
6th meeting, 18th of May 2021
Meeting summary
We have generated a detailed protocol and are currently testing the following parameters: 1) how does bead size affect the coregistration accuracy. We test 200nm, 1µm, and 5µm beads at the moment. 2) are the suggested settings from the iso manual neccessary, or can we generate a more convenient protocol. We test the impact of pixel size and z-step size (16nm/40nm, and 70/200nm).
From first measurements, we have seen that a variation from speciment preparation can be rather high. Thus, one of us (Aurelien) is creating the samples for the rest of WG4.